User manual

146
Bath section (Fig. 6.1.1, top left). The electrode or pipette resistance is read out as Rpipette in Mohms.
Set the appropriate test pulse amplitude by changing the value in the Pulse Amp edit field.
The Sweep Duration can be set between 10 and 1000 msec, and repeat between 10 Hz and 1 Hz (Fig.
6.1.1, bottom left). The test stimulation can be stopped by pressing the “Stop (F4)” button (or pressing
F4) (Fig. 6.1.1, bottom left).
6.2 Forming a Seal
Once the electrode is on the cell surface, click the "Start - Low Pulse Amp (F6)" button (or press F6) to
output the low pulse amplitude pulse to approximately measure the seal resistance, Rseal, in the Patch-
Clamp SealTest Mode / Form Seal section (Fig. 6.2.1, middle left). Once the seal is coming into the
Gohm range, click the "Start - High Pulse Amp (F7)" button (or press F7) to output the high pulse
amplitude to accurately measure the seal resistance, Rseal. Set the appropriate low and high pulse
amplitudes in the neighboring pulse amplitude edit fields.
Fig. 6.2.1. Patch-Clamp Sealtest Protocol / Form Seal after a gigaohm seal has been formed and the
“Start – High Pulse Amp (F7)” button (or F7) has been clicked. Rseal reads out the seal resistance in
Gohms.