User manual

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CHAPTER 6 – Patch-Clamp Sealtest Protocol
A patch-clamp Sealtest Protocol has been implemented for making easier patch-clamp gigohm seal
whole-cell recordings, in particular reading out pipette resistance (Rpipette) when the electrode is in the
bath, seal resistance (Rseal), when forming the seal, and series resistance (Rs), the input resistance
(Rm) and the steady state DC current (Idc) when going/gone whole-cell.
With WinLTP in the Normal Mode, go to Patch-Clamp SealTest Mode by clicking on the "SealTest AD0"
or "AD1" button. In order for these SealTest buttons to appear, AD0 and/or AD1 datatype must be in
"pA" (set by the Edit Protocol dialog box, see Fig. 2.13.1.1).
6.1 Electrode in Bath
Put the patch electrode in the bath. To record the patch electrode or pipette resistance in the bath, click
the Electrode in Bath "Start (F5)" button (or press F5) in the Patch-Clamp SealTest Mode / Electrode in
Fig. 6.1.1. Patch-Clamp Sealtest Protocol / Electrode in Bath after the patch pipette or electrode has
been placed in bath and the “Start (F5)” button (or F5) has been clicked. Rpipette reads out the electrode
resistance in Mohms.