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DuoSpray™ Ion Source User Reference
AB SCIEX TripleTOF
®
5600/5600+ Instruments
96 of 116
System User Guide
D5033331 A
1. Switch to a mobile phase that is 50:50 water:acetonitrile or 50:50 water:methanol.
2. Adjust the position of the TurboIonSpray and APCI probes so that they are as far
from the orifice as possible.
3. In the Analyst TF software, set Temperature (TEM) to between 500 and 600, Ion
Source Gas 1 (GS1) and Ion Source Gas 2 (GS2) to at least 40, and Curtain Gas
(CUR) to the highest setting possible.
4. Wait until the TEM setpoint is reached.
5. Infuse or inject mobile phase through the sample tubing and probe at 1 mL/min for
about 10 to 15 minutes.
6. Make sure that the probe and sample tubing are flushed thoroughly.
Remove the Ion Source
Always remove the ion source from the mass spectrometer before you perform any maintenance
on the ion source or exchange probes.
1. Stop any ongoing scans.
2. Shut down the sample stream.
3. Using the Analyst TF software, put the mass spectrometer in Standby mode. Refer
to the Analyst TF software Help.
4. Let the ion source cool for at least 10 minutes.
5. Disconnect the sample tubing from the grounding union.
6. Turn the two source latches upward to release the ion source.
7. Pull the ion source gently away from the vacuum interface.
8. Put the ion source on a clean, secure surface.
Remove the Probe
1. Remove the ion source from the mass spectrometer. Refer to Remove the Ion
Source.
2. Loosen the 1/8-in. sample tubing nut and remove the sample tubing from the probe.
3. Loosen the bronze retaining ring that fastens the probe to the ion source housing.
4. Gently pull the probe straight up out of the tower. Do not let the tip of the probe touch
anything during removal or storage.
5. Put the probe on a secure, clean surface.
WARNING! Hot Surface Hazard: Surfaces of the ion source become hot during
operation. Let the ion source cool for at least 10 minutes before starting any
maintenance procedures.
WARNING! Electrical Shock Hazard: Disconnect the source from the mass
spectrometer before starting any maintenance procedures.