User guide

DuoSpray™ Ion Source User Reference
System User Guide
D5033331 A
AB SCIEX TripleTOF
®
5600/5600+ Instruments
93 of 116
Caution: Potential Equipment Damage: If the LC system connected to the mass
spectrometer is not controlled by the Analyst TF software, then do not leave the mass
spectrometer unattended while in operation. The LC system can flood the ion source
housing when the mass spectrometer goes into Standby mode.
1. Start the Analyst TF software.
2. In the Analyst TF software, in Tune and Calibrate mode, double-click Manual
Tuning.
3. Set the Temperature (TEM) parameter to 450 and let the ion source warm up for 30
minutes or until the ion source housing is warm to the touch.
The 30-minute warm-up stage prevents solvent vapors from condensing in a cold
probe.
4. Start the sample flow.
5. Run the method to be used to optimize the ion source.
Set the Starting Conditions
1. On the Source/Gas tab in the Tune Method Editor, in the Ion Source Gas 1 (GS1)
field, type 0.
2. In the Ion Source Gas 2 (GS2) field, type 20.
3. In the Curtain Gas (CUR) field, type 20.
4. Type a starting value for IonSpray Voltage Floating (ISVF).
5. On the Compound tab, in the Declustering Potential (DP) field, type 80.
Optimize Gas 1, Gas 2, and Curtain Gas™ Flow
1. Adjust GS1 and GS2 in increments of 5 to achieve the best signal or signal-to-noise
ratio.
2. Increase CUR until the signal starts to decrease.
Note: The value for the GS1 parameter, which is used by the
TurboIonSpray probe, may influence performance of the APCI probe. You
may need to adjust the GS1 parameter value to achieve optimal
performance.
Note: Gas 2 is used as a nebulizer gas for the APCI probe.
Note: To prevent contamination, use the highest value for CUR possible
without sacrificing sensitivity. Do not set CUR lower than 20.