User guide
DuoSpray™ Ion Source User Reference
AB SCIEX TripleTOF
®
5600/5600+ Instruments
92 of 116
System User Guide
D5033331 A
especially if the probe is too low, resulting in signal instability and a high chemical background
noise. Similarly, a high heater gas flow could produce a noisy or unstable signal.
1. Adjust GS1 and GS2 in increments of 5 to achieve the best signal or signal-to-noise
ratio.
2. Increase CUR until the signal starts to decrease.
3. Adjust ISVF in increments of 100 V to achieve the best signal or signal-to-noise ratio.
Optimize the Turbo Heater Temperature
The quantity and type of sample affects the optimal TurboIonSpray probe temperature. At higher
flow rates the optimal temperature increases.
• Adjust the TEM value in increments of 50°C to achieve the best signal or signal-to-
noise ratio.
Optimize for the Compound
• Adjust the DP value in increments of 10 to achieve the best signal or signal-to-noise
ratio. DP optimizes between 60 to 200.
Optimize the APCI Probe
Optimize performance by injecting a known compound and monitoring the signal of the known
ion. Adjust the parameters to maximize the signal-to-noise ratio.
Run the Method
Caution: Potential Equipment Damage: Warm the ceramic heater slowly to avoid thermal
shock to the heating element.
Note: To prevent contamination, use the highest value for CUR possible
without sacrificing sensitivity. Do not set CUR lower than 20.
Note: If the ISVF is too high, a corona discharge can occur. It is visible as
a blue glow at the tip of the TurboIonSpray probe. This will result in
decreased sensitivity and stability of the ion signal.
WARNING! Toxic Chemical Hazard: Vent the source exhaust system to an
external fume hood or an external vent to prevent hazardous vapors from being
released into the laboratory environment.
Table 7-3 Typical Values for Optimizing the APCI Probe
Parameter Typical value Operational range
Probe X-axis position 5 mm Scale 0 mm to 10 mm
Probe Y-axis position 5 mm Scale 0 mm to 13 mm
The optimal X-axis position is within 0 mm to 2 mm on either side of the orifice.
The optimal probe Y-axis position is within 3.0 mm to 7.0 mm of the orifice.