User guide

DuoSpray™ Ion Source User Reference
System User Guide
D5033331 A
AB SCIEX TripleTOF
®
5600/5600+ Instruments
91 of 116
For LC pumps, use a value between 30 and 50 for GS2.
3. Type a starting value for IonSpray Voltage Floating (ISVF).
4. In the Curtain Gas (CUR) field, type 20.
5. On the Compound tab, in the Declustering Potential (DP) field, type 80.
Adjust the TurboIonSpray Probe Position
At low flow rates, the probe can be adjusted to its lowest Y-axis position. For high flow rates,
position the probe higher than the orifice. The curtain plate orifice should remain clear of solvent
or solvent droplets at all times.
For multiply-charged proteins and peptides introduced at a few microliters per minute, position
the sprayer nozzle higher than the curtain plate orifice.
1. Look through the window of the ion source housing to view the position of the probe.
2. Set the X-axis adjustment knob to 5 and the Y-axis adjustment knob to 5.
3. Infuse or inject the sample.
4. Monitor the signal in the software.
5. Use the X-axis adjustment knob to adjust the probe position in small increments until
the best signal or signal-to-noise ratio is achieved.
The TurboIonSpray probe may optimize slightly to either side of the orifice.
6. Use the Y-axis adjustment knob to adjust the probe position in small increments to
achieve the best signal or signal-to-noise ratio.
7. Adjust the black electrode adjustment nut on the probe to move the electrode tip
relative to the sprayer tube. For optimal performance, the electrode should protrude
0.5 mm to 1.0 mm.
After the probe is optimized, it needs only minor adjustment. If the probe is removed,
or if the analyte, flow rate, or solvent composition changes, repeat the optimizing
procedure after installation.
Optimize the Source/Gas Parameters
Optimize nebulizer gas (Gas 1) for best signal stability and sensitivity. The heater gas (Gas 2)
helps in the evaporation of solvent, which helps to increase the ionization of the sample. Too high
a temperature can cause premature vaporization of the solvent at the TurboIonSpray probe tip,
Note: Gas 2 is used with higher flow rates typical with an LC system and in
conjunction with increased temperature.
Note: The vertical position of the probe depends on flow rate. At low flow
rates, the probe should be closer to the orifice. At higher flow rates, the
probe should be farther away.
WARNING! Toxic Chemical Hazard: Make sure that the electrode tip
extends past the end of the probe, to prevent the escape of hazardous
vapor from the ion source.