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DuoSpray™ Ion Source User Reference
AB SCIEX TripleTOF
®
5600/5600+ Instruments
90 of 116
System User Guide
D5033331 A
Optimize the TurboIonSpray Probe
Optimize performance while injecting a known compound and monitor the signal of the known
ion. Adjust the parameters to maximize the signal-to-noise ratio and signal stability.
Caution: Potential Equipment Damage: If the LC system connected to the mass
spectrometer is not controlled by the Analyst
®
TF software, then do not leave the mass
spectrometer unattended while in operation. The LC system can flood the ion source
housing when the mass spectrometer goes into Standby mode.
Run the Method
1. Start the Analyst TF software.
2. In the Analyst TF software, in Tune and Calibrate mode, double-click Manual
Tuning.
3. Set the Temperature (TEM) parameter to 450 and let the ion source warm up for 30
minutes or until the ion source housing is warm to the touch.
The 30-minute warm-up stage prevents solvent vapors from condensing in a cold
probe.
4. Start the sample flow.
5. Run the method to be used to optimize the ion source.
Set the Starting Conditions
1. On the Source/Gas tab in the Tune Method Editor, type a starting value for Ion
Source Gas 1 (GS1).
For LC pumps, use a value between 40 and 60 for GS1.
2. Type a starting value for Ion Source Gas 2 (GS2).
Tip! It is easier to optimize signal and signal-to-noise with FIA or on-column injections.
Note: The IonSpray Voltage Floating (ISVF) is always applied to both the
TurboIonSpray probe and the APCI probe simultaneously, and the Temperature (TEM)
is always applied to both the turbo and APCI heaters simultaneously.
Table 7-2 Typical Values for Optimizing the TurboIonSpray Probe
Parameters LC flow rate Operational
range
5 µL/min to
50 µL/min
200 µL/min 1000 µL/
min
5 µL/min to
3000 µL/min
Probe X-axis position 3 mm to 8 mm 0 mm to 10 mm
Probe Y-axis position 5 mm to 10 mm 0 mm to 5mm 0 mm to 13 mm
The optimal X-axis position is within 0 mm to 3 mm on either side of the orifice.
The optimal Y-axis position is within 3.0 mm to 7.0 mm of the orifice.