User guide

Analyzing and Processing Data
AB SCIEX TripleTOF
®
5600/5600+ Instruments
70 of 116
System User Guide
D5033331 A
There are several methods for extracting ions to generate an XIC, depending on whether
chromatographic or spectral data is used. Table 6-5 contains a summary of methods that can be
used with chromatograms and spectra.
To generate an XIC using a selected range
1. In the Navigation Bar, under Explore, double-click Open Data File.
The Select Sample dialog opens.
2. In the Data Files list, select a file containing spectra.
3. Click OK.
4. To select a range inside the pane, click and hold the left mouse button at the start of
the range and then drag the cursor to the stop point and release.
The selection is highlighted in blue.
5. Click Explore > Extract Ions > Use Range.
An XIC of the specified selection opens in a pane below the spectrum pane. The
experiment information at the top of the pane contains the mass range and the
maximum intensity in counts per second.
Table 6-5 Summary of XIC Generation Methods
Method Use with
chromatogram
Use with
spectrum
Extraction
Selected range No Yes The selected range method extracts
ions from a selected area in a
spectrum.
Maximum No Yes The maximum method extracts ions
from a selected area in a spectrum
using the most intense peak in the
selected area. This creates an XIC
using the maximum mass from the
selected spectral range.
Base peak
masses
Yes No The base peak masses method can be
used only with BPCs (Base Peak
Chromatograms.) Use the Use Base
Peak Masses command to extract ions
results in an XIC with a different
colored trace for each mass. If the
selection includes multiple peaks, the
resulting XIC will have an equal
number of colored traces representing
each mass.
Specified masses Yes Yes The specified masses method extracts
ions from any type of spectrum or
chromatogram. Select up to ten start
and stop masses for which to generate
XICs.