User guide

Analyzing and Processing Data
System User Guide
D5033331 A
AB SCIEX TripleTOF
®
5600/5600+ Instruments
69 of 116
Show TICs from a Spectrum
Prerequisite: The Example project is selected.
1. In the Navigation Bar, under Explore, double-click Open Data File.
The Select Sample dialog opens.
2. In the Data Files list, open a file containing spectra.
3. Click Explore > Show > Show TIC.
The TIC opens in a new pane.
Show a Spectrum from a TIC
1. In a pane containing a TIC, select a range.
2. Click Explore > Show > Show Spectrum.
The spectrum opens in a new pane.
Generate XICs
The user can generate XICs only from single period, single experiment chromatograms or
spectra. To obtain an XIC from multi-period or multi-experiment data, split the data into separate
panes by clicking the triangle that is under the x-axis. For more information about using the
available icons, refer to Table 6-8.
BPC (Base Peak
Chromatogram)
Chromatographic plot that shows the intensity of the most
intense ion within a scan versus time or scan number.
TWC (Total Wavelength
Chromatogram)
A chromatographic display created by summing all of the
absorbance values in the acquired wavelength range and then
plotting the values against time. It consists of the summed
absorbances of all ions in a scan plotted against time in a
chromatographic pane.
XWC (Extracted Wavelength
Chromatogram)
A subset of TWC, an XWC shows the absorbance for a single
wavelength or the sum of the absorbance for a range of
wavelengths.
DAD (Diode Array Detector) A UV detector that monitors the absorption spectrum of eluting
compounds at one or more wavelengths.
Tip! Right-click inside a pane containing a spectrum and then click Show
TIC.
Tip! Double-click in the TIC pane at a particular time to show the
spectrum.
Table 6-4 Chromatograms (Continued)
Types of chromatograms Purpose