User guide

Basic Acquisition Methods
AB SCIEX TripleTOF
®
5600/5600+ Instruments
48 of 116
System User Guide
D5033331 A
1DP
(Declustering
Potential)
Compound The DP parameter controls the voltage
on the orifice, which affects the ability
to decluster ions between the orifice
and QJet
®
ion guide. It is used to
minimize the solvent clusters that may
remain on the sample ions after they
enter the vacuum chamber, and, if
required, to fragment ions. The higher
the voltage, the higher the energy
imparted to the ions. If the DP
parameter is too high, unwanted
fragmentation may occur.
Use the preset value and optimize for
the compound.
TOF MS/MS
2 CE (Collision
Energy)
Compound The CE parameter controls the
potential difference between Q0 and
Q2 (collision cell). This is the amount of
energy that the precursor ions receive
as they are accelerated into the
collision cell, where they collide with
gas molecules and fragment.
Use the preset value and optimize for
the compound.
Q1, MS/MS
2CES
(Collision
Energy
Spread)
Compound The CES parameter, in conjunction
with the Collision Energy (CE),
determines the collision energy applied
to the precursor ion in a Product Ion
scan. The collision energy is ramped
from low to high. For example, in
positive mode, the collision energy will
be ramped from CE – CES to CE +
CES. By entering a CES value,
collision energy spread is automatically
turned on.
Use the preset value and optimize for
the compound.
Q1, MS/MS
Figure 4-1 Ion optics path and parameters (Continued)
Item Parameter Parameter
type
Use Scan type