User guide

Basic Acquisition Methods
System User Guide
D5033331 A
AB SCIEX TripleTOF
®
5600/5600+ Instruments
47 of 116
1 IonSpray
Voltage
Floating
(ISVF)
Source and
Gas
The ISVF parameter affects the
stability of the spray and hence the
signal sensitivity. This is the voltage
applied to the needle that sprays the
sample.
All
1 Nebulizer
Current (NC)
Source and
Gas
The NC parameter controls the current
applied to the corona discharge needle
in the APCI probe used in the
TurboIonSpray
®
ion source when using
the Heated Nebulizer probe. The
discharge ionizes solvent molecules,
which in turn ionize the sample
molecules.
All
1 IHT (Interface
Heater
Temperature)
Source and
Gas
The IHT parameter controls the
temperature of the NanoSpray
®
interface heater and is only available if
the NanoSpray ion source and
interface are installed.
The optimal heater temperature
depends on the type of sample being
analyzed and the solvent used. If the
heater temperature is too high, the
signal degrades. Typically, heater
temperatures are in the 130 to 180 °C
range. The maximum heater
temperature that can be set is 250 °C,
but this is too high for most
applications.
All
2 CAD Gas Compound The CAD parameter controls the
pressure of collision gas in the collision
cell. The collision gas helps to focus
the ions as they pass through the
collision cell; the preset for the CAD
parameter is in fixed mode. For MS/
MS-type scans, the collision gas aids in
fragmenting the precursor ions. When
the precursor ions collide with the
collision gas, they can dissociate to
form product ions.
Use the preset value and optimize for
the compound.
All
Figure 4-1 Ion optics path and parameters (Continued)
Item Parameter Parameter
type
Use Scan type