User guide

System User Guide
D5033331 A
AB SCIEX TripleTOF
®
5600/5600+ Instruments
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Basic Acquisition Methods
An acquisition method consists of the method for the mass spectrometer and for liquid
chromatography (LC) devices. Users can easily create an acquisition method using the Method
Wizard.
The Acquisition Method Editor can also be used to create acquisition methods and to add a
sequence of periods and experiments for the instrument and devices.
Users can use the SWATH™ acquisition feature, available in both the Method Wizard and the
Acquisition Method Editor, to create SWATH acquisition methods. For more information, refer to
the Advanced User Guide.
Create an Acquisition Method using the Method
Wizard
The acquisition method can be saved in an existing project.
1. Make sure that a hardware profile containing the mass spectrometer and peripheral
devices is active.
2. On the software toolbar, make sure that the appropriate project is selected.
3. On the Navigation bar, in Acquire mode, double-click Method Wizard.
The Method Wizard appears.
4. From the Choose MS Method list, select TOF MS (+).
5. From the Choose LC Method list, select the LC method that was created for the
hardware profile.
6. Type a name for the method and then press Enter.
7. Click Next.
8. On the Ion Source Parameters tab, verify the values, editing them if necessary, and
then click Next.
9. On the TOF MS tab, verify the values, editing them if necessary, and then click
Finish.
Tip! To copy the Method Wizard template methods into the Acquisition Methods
folder in the project folder, select the Copy method templates check box in the Create
New Project or Subproject dialog. To open this dialog, click Tools > Project > Create
Project or Create Subproject.
Tip! Move the cursor over the interface to view tool tips and procedures.