User Manual

8. Procedure

• Open two sealed cassettes and remove the 150ul transfer pipets. Perform A and B for each

sample evaluation.

Sample:

o Using the transfer pipet remove 150 μL of sample from the 125mL water sample bottle

o Transfer 150 μL of the sample into one of the sample dilution buffer tubes

o Mix by pipetting up and down 3 times.

o Using the transfer pipet add the diluted sample directly into the sample port of the

cassette.

Negative Control:

o Use the transfer pipet to transfer 150 μL of Negative Control into the sample port of

the cassette.

o Incubate for 15-30 minutes.

o Read results.

9. Interpretation of Results

For samples prepared as described above, screening concentrations are determined by comparison of the

intensity of the test line to the intensity of the control line on parallel test strips. Although control line

intensity may vary, a visible control line must be present for results to be considered valid. Test strips

with a test line which is darker than or of equal intensity to the test line of the control indicates a

result which is below the limit of detection of the test. Test strips with a test line which is lighter than

the control strip indicates a result which is ≥ 5 ppb. Test strips with no test line visible (only the

control line is visible) indicates a result which is ≥ 50 ppb. Results should be determined within 15-30

minutes after completion of the strip test procedure. Determination made using strips which have dried

for more or less than the required time may be inaccurate, as line intensities may vary with drying

time.