User`s guide

Chapter 4: Running Samples 127
4 Replace the container cap and hand-tighten it until it is fully closed.
5 If you disconnected the sensor and quick-release connectors in step 1:
a Reconnect the sensor and quick-release connectors to their respective
ports.
b Prime the fluidics system.
From the BD FACSDiva menu bar, select Cytometer > Cleaning
Modes > Prime after Tank Refill. In the dialog box, select the fluid to
prime, then click OK.
Emptying the Waste Container
Empty the waste container daily and when the fluid indicator shows the waste is
getting full. To prolong the life of the container, we recommend that you switch
to the alternate container each time the waste is emptied.
To e nsure that the appropriate solutions are dispe nsed, do not switch the
tank positions. Make sure the label on each container matches the labelled
port on the fluidics cart.
All biological specimens and materials coming into contact with them can
transmit potentially fatal disease. To prevent exposure to biohazardous
agents, expose waste container contents to bleach (10% of total volume)
before disposal. Dispose of waste in accordance with local regulations. Use
proper precautions and wear suitable protective clothing, eyewear, and
gloves.
Change the waste container cap every month to prevent container
pressurization. To order new replacement caps (BD Part No. 33885407,
pack of 12), contact your local BD Biosciences representative.