Specifications

CHAPTER 11 Troubleshooting guide 73
CHAPTER 11
Troubleshooting guide
No detector response
Possible cause Remedy
No power Check line voltage setting, plug in power
cord
Power switch off Turn this switch ‘on’ (at the rear panel)
Faulty fuse Replace fuse
Divergent mains voltage Check line voltage, see page 13
Cell disconnected, or switched off Check connection
Recorder/integrator disconnected Check connection
Fouled WE Clean WE (see flow cell manual)
High cell current
Possible cause Remedy
Contaminated buffer Replace buffer, do not recycle the buffer
High WE potential Optimise potential, if possible: use smaller
WE diameter
Salt bridge in REF not saturated Refill with wetted KCl crystals
Retained peaks from previous
runs
Wait for elution of these (very) broad
peaks
Column is ‘bleeding’ Replace column
High amount of Fe
++
in buffer Add EDTA to buffer, rinse metal parts with
15% HNO
3
Noisy baseline
Possible cause Remedy
Salt bridge in REF not saturated Refill with saturated KCl, add wetted KCl
crystals
Air bubble in REF or in cell Remove air bubble, continuously degas
the mobile phase
Slow temperature fluctuations Isolate detector cell, set oven temperature
Fouled WE Clean WE (see flow cell manual)
Leaking REF or cell Tighten connections with care
Decreased sensitivity (low S/N ratio)
Possible cause Remedy
Fouled WE by dirty samples Clean WE (see flow cell manual), if
possible: dilute samples
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