Operator’s Manual DaisyII Incubator D200, D200I 2052 O’Neil Rd Macedon NY 14502 Telephone: (315) 986-8090 · Fax: (315) 986-8091 www.ankom.
TABLE OF CONTENTS Introduction. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3 Safety Precautions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 Warranty. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4 Instrument Description and Setup. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Operation. . . . . . . . . . . . . . . . . . . . . . . . . .
Congratulations on your purchase of an ANKOM Te c h n o l o g y DaisyII Incubator. Your selection of an ANKOM system indicates that you are looking to increase your wet chemistry efficiency and maintain the highest levels of precision and accuracy possible. The DAISYII Incubator was designed to efficiently and accurately analyze up to 100 samples individually enclosed in filter bags. You can perform either rate studies or total digestion studies in a batch operation.
Safety Precautions Caution, Lift Hazard- Lift and move using two people only. Rear Surface: Caution, Hazardous Voltages- Do not operate the instrument with the back cover removed - hazardous voltages are present during operation. The power cord must be connected prior to rear panel removal. Electrical shock or electrocution may result if ignored. General: All switches should be in the off position before plugging the power cord into the electrical outlet.
Instrument Description and Set-up: Your instrument comes complete with a power cord and digestion jars. - Set the instrument on a firm, level surface. Place the back of the instrument no closer than one inch from a wall. NOTE: Do not locate this instrument where it will be subject to excessive shock, vibration, dirt, moisture, oil, or other fluids. Plug the power cord into the plug outlet and then into an electrical outlet. The Rotate button controls the rotation of the digestion jars.
Operation Your DAISYII Incubator is designed to provide incubation and agitation of samples at a constant 39.5C+/-.5. The controller is preset to 39.5°C. Consult ANKOM Technology if a different temperature setting is desired. To Start the Cycle: 1) Turn the instrument’s Main Power Switch on. 2) Add samples and solution to digestion jars as per procedure. 3) Turn the Heat and Rotate buttons on. Visually confirm the jars are rotating and the heat lamps are on.
Troubleshooting (Continued) Problems (Continued): Problem: The temperature controller reading does not match the actual temperature. The temperature controller reading is low. Cause: A bulb is not working, the air flow is obstructed. Solution: Make sure both heat bulbs are working. Check for an obstruction of hot air flow. Make sure the Daisy is not under a cold air vent. If this does not solve the problem, contact ANKOM Technology. Problem: Controller reads “no load.
Appendix A 8 W-1 ANKOM Technology Fiber Weighing Procedure For In Vitro Determinations Electronic BalanceWe recommend a four place readout on the balance and suggests “Balance Talk™” or other LIMS software for data input and management. Heat Sealer # 1915 (100V - 120V) # 1920 (220V - 240V) Sample Filter Bags # F57 MoistureStop Weigh Pouch # X45 1. Wash and dry filter bags as per procedure. Number all bags using a solvent resistant marker. 2. Weigh and record filter bag weights.
Appendix A (Continued) 9 W-1 ANKOM Technology Fiber Weighing Procedure (Continued) For In Vitro Determinations 4. Add 0.25g to .05g of sample to bag using a spatula. Be careful not to get sample particles on top edge of the bag sealing area. 7. Seal each filter bag no more than 4mm from it’s open end. Hold down handle for 2-3 seconds after the red light turns off. 9. Perform the extraction as per procedure. 5. Weigh sample and record weight 6. Heat sealer dial should be set between 4 and 5.
Appendix B 10
Appendix C Daisy Assemblies and Parts D1.5 - Temperature Probe D2 - Jar Assembly D2.
Appendix C (Continued) 12 Daisy Assemblies and Parts (Continued) 100.
Appendix D 13 ANKOM Technology Method 3 II In Vitro True Digestibility using the DAISY Incubator P ANKOM T e c h n o l o g y P - 08/05 A. Reagents (a) UBuffer Solution A:U KHB2BPOB4B MgSOB4B•7HB2BO NaCl CaClB2B•2HB2BO Urea (reagent grade) Ug/literU 10.0 0.5 0.5 0.1 0.5 (b) UBuffer Solution B: NaB2BCOB3B NaB2BS•9HB2BO 15.0 1.0 (c) Neutral Detergent Solution B. Apparatus (a) (b) (c) (d) (e) DAISYPIIP Incubator Filtration device - F57 Filter Bags Impulse bag sealer - 1915/1920 Heat Sealer.
Appendix D (continued) 14 Preparation of Inoculum and Incubation: Maintain all glassware at 39°C (a) Preheat two 2L thermos bottles by filling with 39° C water. Empty heated water just prior to collection of rumen inoculum. Using the appropriate collection procedure, remove at least 2000 ml of rumen inoculum and place in thermos. Include approximately two "fistfuls" of the fibrous mat from the rumen with your collection in one thermos. (b) Preheat a blender by filling with 39PoP C water.
Appendix D (continued) 15
Appendix E Daisy Wiring Diagram Inlet Power: 100-120V 220-240V 16
