User guide


Workaround:Theduplicatewarningmessagesarethesame.Clickingoneitherofthemessageswill
bringupthedialogboxforthecorrectionaction.
Incorrect highlighting of the Type II RE cloning Sequence
Issue:TypeIIREcloningSequenceishighlightedincorrectlyontheselectedcutrestrictionsiteoverhang
featureonVectorSelectiondialog.
Workaround:ThecorrectOverhangsequenceofthefeatureisdisplayedintheareaundertheSequence
ViewerpaneinthePrepareVectordialogbox.Thecorrectlyhighlightedfeaturecanalsobeviewedinthe
PreviewResultsdialogboxforthevectorbackboneafterassembly.
The default assembly name is not set correctly by the software
Issue:IntheSeamless/HighOrder(Liang,2012)andGoldenGate(Engler,2011)assemblytools,ifthe
Configuredialogisinvokedmultipletimes,andpartsareaddedtothedevicebeingconfiguredduring
theinvokes,onsomeoccasionsthedefaultvalueintheNamefieldintheConfiguredialogdoesnot
displaythecorrectpartsthathavebeenselected.
Workaround:Ensurethatyoualwaysdouble‐checktheNamebeforeperformingthecloning.
Sequences for features in the complementary strand are not highlighted in the Assembly
Compatibility tool
Issue:IntheAssemblyCompatibilitytool,intheVectorSelectiondialogbox,iftheuserclicksona
featureinthecomplementarystrand,thefeatureisnothighlightedintheSequenceViewerpane.
Workaround:Manuallyhighlightthesequenceusingthestartandendpositionshowninthetooltipin
Graphicpane.
The Part category field is defaulted to blank upon re-importing an exported Device
Issue:ThePartCategoryofanexportedPartisnotshownasthedefaultvalueintheImportdialogboxif
thispartisre‐importedtotheVectorNTI
®
ExpressDesignerSoftwareaftertheDevicecontainingthatPart
isexported.
Workaround:AlwayscheckthePartCategorywhenimportingaPartbackintothesoftware.
Dot Plot is sometimes hidden behind the RNA Analysis dialog box
Issue:SometimestheDotPlotdialogboxishiddenbehindtheRNAAnalysisdialogboxandcanbe
invisibletotheuser.
Workaround:PressAlt+TabtobringuptheDotPlotdialogbox.
RBS Analysis and RBS Design dialog boxes cannot be maximized
Issue:TheRBSCalculatordialogboxescannotberesizedtherebymakingitdifficulttoexaminethe
results.
Workaround:ClicktheExportReportbuttontoexporttheresultstoMicrosoft
®
Excel
®
,andexaminethe
resultsfromthere.
System cannot refresh the Organism list (in Mac versions only)
Issue:IntheRBSCalculatorAnalysistool,whentheusertypesonlythreelettersintheOrganismfield,
thedrop‐downlistdoesnotrefresh.ThishappensonlyintheMacintosh
®
versionoftheVectorNTI
®

ExpressDesignerSoftware.
Workaround:Clickinthedrop‐downlistmanuallyafterkeyinginthe3letters;thelistwillgetrefreshed.

Vector NTI
®
Express Designer Installation and Licensing Guide 5