User manual

5 Trouble-shooting
5.1 General
When contacting Amersham Biosciences for support, state
the program version of the module, which is shown for 2 seconds
during start-up.
5.2 Faults and actions
If the suggested actions do not correct the fault, call Amersham
Biosciences.
UV measurement
No text on the front display 1 Check that the mains cable is connected and the power
switch is in ON-position I.
Noisy UV-signal
drift or instability 1 Select menu Check Autozero to check the autozero
absorbance value. If AZ is between 1.5 and 2, there may be
air bubbles in the flow cell, or the wrong buffer system in
use.
2 Wrong filter for the lamp used. Check that the lamp is in
proper position and that the correct filter is used.
3 The buffer may be impure. Check if the signal is still noisy
with water.
4 There may be air in the flow cell. Check that the flow
restrictor gives a back-pressure of 0.2 MPa.
5 If there is a lot of air in the water, degas the buffer before
use.
6 Check the connections of the optical unit.
7 Clean the UV-cell, see sections 4.3 and 4.4.
8 Locking nut in optical unit not properly tightened. Turn the
locking nut to the stop position.
Ghost peaks 1 Check that there is no air in the eluents. Degas if necessary.
2 Clean the system in accordance with the ÄKTA
FPLC
System Manual.
3 Clean the column in accordance with the column
instructions.
4 Check that the mixer is functioning correctly and that the
correct chamber volume is being used.
WARNING! The module must not be opened by the user. It
contains high voltage circuits that can deliver a lethal electric
shock.
UPC-900
Version 1.00
Monitor UPC-900
User Manual 18-1125-55 Edition AE
27
Trouble-shooting 5