User manual

Monitor UPC-900
User Manual 18-1125-55 Edition AE
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When necessary, replace the lamp according to Section
4.10 Changing the UV lamp, or contact Amersham Biosciences for
lamp replacement.
Autozero
The module internal absorbance value for autozero can be checked
to test the consistency of buffers.
1 Select menu
Check and press OK.
2 Select menu
Check Autozero. The autozero absorbance value
for the wavelength used is shown.
4.3 Cleaning the UV flow cell in-place
Pump a cleaning or sanitising agent through the flow cell. The
standard recommendation is to pump 1 M NaOH for 30 minutes
and then wash out with buffer.
4.4 Cleaning the UV flow cell off-line
A clean flow cell is essential for ensuring the correct operation of the
UV-monitor.
1 Connect a syringe to the inlet of the flow cell and squirt distilled
water through the cell in small amounts. Then fill the syringe
with a 10% surface active detergent solution like Decon 90,
Deconex 11, RBS 25 or equivalent, and continue to squirt five
more times.
2 After five squirts, leave the detergent solution in the flow cell for
at least 20 minutes.
3 Pump the remaining detergent solution through the flow cell.
4 Rinse the syringe and then flush the flow cell with distilled water
(10 ml).
CAUTION! Do not allow solutions that contain dissolved salts,
proteins or other solid solutes to dry out in the flow cell. Do not
allow particles to enter the flow cell as damage to the flow cell
may occur.
WARNING! NaOH is injurious to health. Avoid spillage.
Check Autozero
Maintenance 4