User manual

2.5 Installing the pH flow cell and electrode
Mounting the flow cell holder
In ÄKTA
FPLC systems, the pH electrode is optional.
1 Hook the flow cell holder on the right hand side of the housing.
Secure it with the slide clamp.
If the flow cell holder is not used, the flow cell must
still be installed at an angle of 30° from the vertical
with the outlet placed higher than the inlet to
prevent air bubbles being trapped in the cell.
The flow direction is marked on the flow cell.
2 Connect the tubing with finger-tight connectors.
Inserting the pH electrode
Note: Handle the pH electrode with care.
1 Unpack the pH electrode. Ensure that it is not broken or dry.
2 Before using the electrode, remove the electrode end cover and
immerse the glass bulb in buffer for 30 minutes.
3 Remove the dummy electrode from
the flow cell and store it in the
flow cell holder.
4 Carefully insert the electrode in the
flow cell. Tighten the nut by hand
to secure the electrode.
Note: If the flow cell is full of
liquid, it is not possible to insert
the electrode. If so, loosen the inlet
connection while inserting the
electrode to allow the liquid to run
out from the flow cell. Remember
to re-tighten the connector.
Note: If the electrode is not fully
inserted, the system will leak and a
dead volume will occur in the holder.
5 Connect the pH electrode cable to the socket
pH Probe on the
rear of the module.
CAUTION! The tip of the pH electrode consists of a thin glass
membrane. Protect it from breakage, contamination and drying
out or the electrode will be destroyed. Always store the electrode
with the end cover filled with a 1:1 mixture of pH 4 buffer and
2 M KNO
3
. Do NOT store in water only.
Monitor UPC-900
User Manual 18-1125-55 Edition AE
7
Installation 2
End
cover
Electrode
To module
“pH probe”
socket
Flow direction
Flow cell
holder
Flow cell
Dummy