User's Manual

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● Choose the appropriate bioprinting probe for different multi-well plate. For example, use the probe
with small tip for the 24-well plate, the probe with medium tip for the 12-well plate, and the probe
with large tip for the 6-well plate.
● The new multi-well plate is sterile and comes in a sterile plastic bag. The new multi-well plates can be
used directly for bioprinting.
● To use the multi-well plate, remove the lid of the multi-well plate and load the multi-well plate to the
plate holder of the BIONOVA X
TM
Model 3 D printer. Make sure the multi-well plate is mounted to the
plate holder properly, tightly, and well leveled.
Warning: Inappropriate mounting of the multi-well plate may cause the failure of the printing or
even damage of the printer.
● After use, remove the multi-well plate off the plate holder of the printer.
5.3. Bioinks
Allegro 3D provides a whole series of Stemink
TM
bioinks with various material properties. Please visit our
website at https://allegro3d.com for details and application notes of the Stemink
TM
bioinks products.
Before printing on BIONOVA X 3 D printer, load appropriate amount of Stemink
TM
bioink solution to our
multi-well plate. Please refer to the table in Section 4.3 for maximum bioink load volume for each type
of well plate. Overloading of the bioink solution will cause spills. Protect bioink solution from light when
it is not used in printing.
Make sure the bioink solution is sterile before mixing with cells for cell-laden structure printings.
We do not recommend reusing the excess bioink solutions left in the multi-well plate after bioprinting.