Agilent G1978B Multimode Source for 6100 Series Single Quad LC/MS User Guide Agilent Technologies
Notices © Agilent Technologies, Inc. 2006 Warranty No part of this manual may be reproduced in any form or by any means (including electronic storage and retrieval or translation into a foreign language) without prior agreement and written consent from Agilent Technologies, Inc. as governed by United States and international copyright laws. The material contained in this document is provided “as is,” and is subject to being changed, without notice, in future editions.
In This Guide This guide explains how to install, maintain and troubleshoot your nanoelectospray ion source. 1 Basic Operation and Maintenance This chapter describes basic operation and maintenance for the multimode ion source. 2 Installation This chapter tells you how to install the multimode ion source. 3 Reference This chapter contains an overview of the multimode ion source, safety precautions, and technical specifications.
Multimode Source for 6100 Series Single Quad LC/MS User Guide
Contents Content 1 Basic Operation and Maintenance 7 Setting up Methods 8 To setup a method to use the multimode source 8 To create a method for positive/negative mixed mode operation To create a method for alternating ESI and APCI operation 11 9 Maintenance 13 To open the multimode source 13 To turn off the instrument 15 To clean the multimode source daily 16 To clean the multimode source weekly 19 To bake out the LC/MS 21 To do an autotune 22 2 Installation 23 Step 1.
Contents Source comparison in negative ion mode 56 Single analysis in MM-ES+APCI mode with fast switching 57 Guidelines 58 Guidelines for multimode source settings in MM-ES mode 58 Guidelines for multimode source settings in MM-APCI mode 59 Guidelines for multimode source settings in MM-ES+APCI mode 60 Guidelines for positive/negative polarity switching in mixed mode 61 Empirical Formulas for high resolution MS 63 G2421A MM-ES Calibration Ions 63 G2422A MM-APCI Calibration Ions 64 Tuning Mix and Test Mix
Agilent G1978B Multimode Source for 6100 Series Single Quad LC/MS User Guide 1 Basic Operation and Maintenance Setting up Methods 8 To setup a method to use the multimode source 8 To create a method for positive/negative mixed mode operation 9 To create a method for alternating ESI and APCI operation 11 Maintenance 13 To open the multimode source 13 To turn off the instrument 15 To clean the multimode source daily 16 To clean the multimode source weekly 19 To bake out the LC/MS 21 To do an autotune 22 Thi
1 Basic Operation and Maintenance Setting up Methods Setting up Methods This section describes the tasks that you need to do to set up methods for your multimode source. To setup a method to use the multimode source To have your method use a multimode source, follow these steps: 1 Open the MSD Spray Chamber dialog box by clicking Instrument > MSD Spray Chamber in the Method and Run Control view. 2 Set Method Spray Chamber to MM-ES+APCI. 3 Verify that Installed Spray Chamber is set to MM-ES+APCI.
Basic Operation and Maintenance To create a method for positive/negative mixed mode operation 1 To create a method for positive/negative mixed mode operation 1 Open the MSD Spray Chamber dialog box by clicking the Instrument > Set Up MSD Signals in the Method and Run Control view. 2 Select MM-ES_APCI from the Method Spray Chamber drop-down list. 3 Check that Installed Spray Chamber is also set to MM-ES+APCI. 4 Make any other changes that are necessary for your method. 5 Click the OK button.
1 Basic Operation and Maintenance To create a method for positive/negative mixed mode operation Polarity options for Signal 1 and Signal 2 Figure 1 10 Positive/Negative polarity switching using Set Up MSD Signals dialog box Multimode Source for 6100 Series Single Quad LC/MS User Guide
Basic Operation and Maintenance To create a method for alternating ESI and APCI operation 1 To create a method for alternating ESI and APCI operation 1 Open The MSD Spray Chamber dialog box by clicking Instrument > MSD Spray Chamber in the Method and Run Control view. 2 Set Method Spray Chamber to MM-ES+APCI. Figure 2 Method Spray Chamber set to MM-ES+APCI. 3 Check that Installed Spray Chamber is also set to MM-ES+APCI. 4 Make any other changes that are necessary for your method. 5 Click the OK button.
1 Basic Operation and Maintenance To create a method for alternating ESI and APCI operation 9 Click the OK button. NOTE In general, use the mixed mode operation (MM-ES+APCI setting with Signal 1) instead of alternating MM-ES (Signal 1) and MM-APCI (Signal 2) mode switching. Two times as many scans are obtained during elution of a chromatographic peak, and no delay is needed between scans, resulting in better data.
Basic Operation and Maintenance Maintenance 1 Maintenance To open the multimode source Open the multimode source to access the end cap and the capillary cap for cleaning and inspection. WA R N I N G Do not touch the multimode source or the capillary cap. They may be very hot. Let the parts cool before you handle them. WA R N I N G Never touch the source surfaces, especially when you analyze toxic substances or when you use toxic solvents.
1 Basic Operation and Maintenance To open the multimode source 7 In the LC/MS ChemStation software MSD Manual Tune view, check that the system recognizes the source. The Source text box in the toolbar should read MM-ES+APCI. Figure 4 List of Modes in Manual Tune View The source listed is the source currently installed on the LC/MS instrument. If the G1978B multimode source is detected, it will indicate MM-ES+APCI. The Mode is the ionization mode you have currently selected.
Basic Operation and Maintenance To turn off the instrument 1 To turn off the instrument Before you begin, check that: • You have completed tuning and data acquisition and you have saved all data as needed. Follow these steps to vent and completely power the instrument off. 1 Use the MSD Vent dialog box to vent the MSD instrument. In the Diagnosis View, select Maintenance > MSD Vent WA R N I N G The quadrupole cools very slowly and will likely be near 100°C when the vent cycle is complete.
1 Basic Operation and Maintenance To clean the multimode source daily To clean the multimode source daily You should clean the multimode source daily or any time you suspect carryover (contamination) from one sample or analysis to another. The multimode source spray chamber is made of 316 stainless steal which is the same material the spray shield is made of.
Basic Operation and Maintenance To clean the multimode source daily WA R N I N G 1 Some mobile phases are hazardous chemicals. Use caution that is appropriate for the current mobile phase. 5 Wipe the interior of the spray chamber with a clean, lint-free cloth. WA R N I N G There are sharp edges inside the spray chamber such as the separator. Pay close attention when wiping the interior of the spray chamber. 6 Rinse the area around the spray shield. Do not spray directly at the end of the capillary.
1 Basic Operation and Maintenance To clean the multimode source daily 11 Close the spray chamber. NOTE 18 If symptoms of contamination persist, or if the spray shield or capillary cap show significant discoloration that cannot be removed by the regular, daily cleaning, use the weekly cleaning procedure.
Basic Operation and Maintenance To clean the multimode source weekly 1 To clean the multimode source weekly The cleaning procedure for cleaning the multimode source weekly is similar to the procedure for cleaning the source daily. The main difference is that the multimode source is removed from the instrument.
1 Basic Operation and Maintenance To clean the multimode source weekly 6 Remove the spray shield. Use abrasive paper to gently clean the end of the capillary cap. 7 Dampen a clean cloth and wipe the end of the capillary cap. 8 Reinstall the spray shield. 9 Use abrasive paper to gently clean the spray shield. Dampen a clean cloth and wipe the spray shield. 10 Rinse the area around the spray shield. Then wipe the area around the spray shield. 11 Reinstall the spray chamber on the instrument.
Basic Operation and Maintenance To bake out the LC/MS 1 To bake out the LC/MS You will need to bake out the LC/MS the first time you turn the system on and any time that the system has been vented. 1 Connect the LC/MS inlet tubing (p/n 0890-1915). 2 Select the Tune view. 3 Select Tune > Manual Tune 4 Display the MS Tune Spray Chamber dialog box. 5 Set the drying gas flow to 7 L/min for MM-ES or 5 L/min for MM-ES+APCI or MM-APCI. 6 Set the nebulizer pressure to 40 psi.
1 Basic Operation and Maintenance To do an autotune To do an autotune Tuning the multimode source is done in MM-APCI mode only. Autotune is performed from the same menu as with all sources. • From the MSD Tune view, select Instrument > Autotune menu item. The tune report will have a header with either the title MM-APCI Positive Mode - Standard Scan or MM-APCI Negative Mode - Standard Scan. You can run the check tune after autotuning to validate that the instrument is passing check tune criteria.
Agilent G1978B Multimode Source for 6100 Series Single Quad LC/MS User Guide 2 Installation Step 1. Prepare to install 24 Step 2. Install the HV control PCA and cables 25 To remove the multimode source 29 To convert from multimode to ESI, APCI or APPI 30 To convert from ESI, APCI or APPI to the multimode source 31 This chapter contains instructions to install the multimode source on a 6100 Series Single Quad LC/MS system, and also to remove and replace the source.
2 Installation Step 1. Prepare to install Step 1. Prepare to install The Multimode Enablement Kit, G1978-60451, is shipped with the multimode source. This kit needs to be installed before the multimode source is used. Note that the multimode source and its accessories are to be installed by an Agilent Customer Engineer.
Installation Step 2. Install the HV control PCA and cables 2 Step 2. Install the HV control PCA and cables 1 Turn off the system power and remove the system power cord. The power cord should be kept intact if the vacuum control switch box is used. The switch box is intended to keep the vacuum on while a CE works on the electronics. The switch box is for CE use only. 2 Remove the CDS cover, top, side, front, and the Aux Module cover.
2 Installation Step 2. Install the HV control PCA and cables 6 Install the APCI HV power supply. The APCI HV power supply is located at the end of the AUX Module. 7 Connect ribbon cable between valve PCA and Vcap/Vchamber power supply. Figure 11 Connecting the valve PCA to the Vcap/Vchamber power supply. 8 Connect the Vcap and Vchamber cables to the Vcap/Vchamber power supply. Figure 12 Connecting the Vcap and Vchamber cables to the power supply.
Installation Step 2. Install the HV control PCA and cables Figure 13 2 Connecting the APCI HV power supply to the valve PCA. 10 Insert one end of the APCI Needle Interlock cable, G1960-60856, through the slot at the front of the system and then plug it to the APCI HV connector. Attach the other end to the chassis with the o-ring and the nut. Figure 14 Connecting the APCI HV to the chassis. 11 Insert the cable, G1960-60858, to the top slot and attach it to the chassis.
2 Installation Step 2. Install the HV control PCA and cables Figure 16 Installing the multimode source (left) and connecting all connectors. 14 Put back the side, top, front and CDS cover. 15 Plug the system power cord back on and turn the front switch on. The pump down process will start. 16 Start the ChemStation program. 17 Click Method and Run Control View > MSD > Spray Chamber and verify that the source is an MM Source. 18 Go to MSD Tune window and start the Auto tune with MM source.
Installation To remove the multimode source 2 To remove the multimode source Do the following steps to remove the multimode source. 1 Set the source temperatures for vaporizer heater and drying gas heater to minimum values to cool the source. Use the Tune > Instrument > Edit Spray Chamber menu item to display the Edit Spray Chamber dialog box. The drying gas flow, nebulizer gas flow, drying gas temperature and the vaporizer temperature should be set to minimum values.
2 Installation To convert from multimode to ESI, APCI or APPI To convert from multimode to ESI, APCI or APPI WA R N I N G Never touch the source surfaces, especially when you analyze toxic substances or when you use toxic solvents. The source has several sharp pieces which can pierce your skin including the APCI corona needle, vaporizer sensor and counter current electrode.
Installation To convert from ESI, APCI or APPI to the multimode source 2 To convert from ESI, APCI or APPI to the multimode source CAUTION If you are installing this source on this instrument for the first time, follow the steps in “Installation” on page 23. 1 Switch to the MSD Tune view. 2 Select Instrument/Set Spray Chamber and set all gas flows and temperatures to 0.
2 Installation To convert from ESI, APCI or APPI to the multimode source Standard spray shield Capillary cap Figure 17 Standard spray shield and capillary cap for ESI or APCI 10 Remove the capillary cap. If needed, moisten a clean cloth with isopropyl alcohol and wipe the capillary cap. See Figure 18. Capillary cap Figure 18 Spray shield removed. 11 Place the capillary cap back on the capillary. 12 Install the new spray shield with field shaping electrodes. See Figure 19.
Installation To convert from ESI, APCI or APPI to the multimode source Figure 19 2 Multimode spray shield 13 Screw the multimode spray shield into the holder for the spray shield. See Figure 20. Field shaping electrode 9 o'clock position Field shaping electrode 6 o'clock position Figure 20 NOTE Multimode spray shield installed The field shaping electrodes should be in the nine o’clock and the six o’clock position.
2 Installation To convert from ESI, APCI or APPI to the multimode source Figure 21 Multimode Spray Chamber 15 Install the spray chamber on the spray chamber mount. I-Button Figure 22 Multimode source with I-Button 16 Install the nebulizer on the multimode source spray chamber.
Installation To convert from ESI, APCI or APPI to the multimode source Figure 23 2 No nebulizer on top of the multimode source 17 Connect the 1/8-inch nebulizer gas tubing from the LC/MS mainframe to the nebulizer gas fitting. See Figure 24.
2 Installation To convert from ESI, APCI or APPI to the multimode source 18 Connect the LC/MS sample tubing to the LC/MS diverter valve inlet filter. See Figure 25 on page 36. WA R N I N G The LC/MS Liquid Chromatograph diverter valve is an integral part of the G1978B safety system. The LC mobile phase flow must always be connected to the diverter valve inlet filter. Never bypass the diverter valve and connect directly to the nebulizer.
Agilent G1978B Multimode Source for 6100 Series Single Quad LC/MS User Guide 3 Reference Safety and Specifications 38 Safety 38 Safety Symbols 42 Technical Specifications 43 Overview 44 Benefits of a multimode source 44 Mobile phase considerations when using the multimode source 52 Flowchart summarizing parameter choices for different modes 54 Source comparison in positive ion mode 55 Source comparison in negative ion mode 56 Single analysis in MM-ES+APCI mode with fast switching 57 Guidelines 58 Guideline
3 Reference Safety and Specifications Safety and Specifications This section describes safety, safety symbols, and technical specifications. Safety Some of the procedures in this chapter require access to parts of the instrument and multimode source while it is in Shutdown state or shortly after it is turned off. If you do not perform these procedures correctly, you are exposed to dangerous temperatures, voltages, and chemical hazards. This topic describes the potential dangers.
Reference Safety 3 • The Infrared medium-wave twin tube emitters are made of quartz glass. The IR radiation is not hazardous and is radiated as heat. • Do not touch any surfaces in the source spray chamber. The spray chamber in most cases will be very hot Hazardous voltages Whenever the instrument is not in Standby, hazardous voltages are present on one or more interior parts. Parts that use hazardous voltages include, but are not limited to, the capillary cap and counter electrode.
3 Reference Safety • Fluid drained from the multimode chamber is made of solvent and sample from your analyses. The fluid in the mechanical pump collects traces of samples and solvents. In addition, unnebulized solvent and sample collects at the bottom of the spray chamber. Connect the drain on the bottom of the spray chamber to a closed container. • Handle and dispose of all fluids using precautions appropriate for their biohazardous and biological content. Comply with local environmental regulations.
Reference Safety 3 Cleanliness Cleanliness and the prevention of accidental contamination during maintenance are very important. Contamination of the interior of the vacuum system or the sample path can affect the results of your analyses. • Always wear clean gloves when handling parts that come in contact with the sample path. Oil from your fingers is difficult to remove. • When you set parts down, place them on clean, lint free cloths or clean aluminum foil, not directly on the laboratory bench.
3 Reference Safety Symbols Safety Symbols NOTE This symbol is placed on the product where it is necessary for you to refer to the manual in order to understand a hazard. WARNING CAUTION This symbol is placed on the product within the area where hazardous voltage is present or shock hazard can occur. Only trained service persons should perform work in this area. WARNING This symbol is placed on the product within the area where hot parts and surfaces are present.
Reference Technical Specifications 3 Technical Specifications Size Weight Power source Height Length Width 17 cm 18 cm 9.5 cm 6.8 in 7.1 in 3.7 in 2.27 kg / 5 lbs. Power 60 VA Primary Voltage 100 V to 240 V Current 0.85 A to 0.40 A Frequency 50 Hz / 60 Hz Secondary Operating Temperature Voltage 12 V Current 3.3 A 15°C to 35°C (59°F to 95°F) (Analytical specifications will be met only within a temperature range of 21°C ± 3°C (70°F ± 6°F).
3 Reference Overview Overview Benefits of a multimode source Figure 26 Multimode source. The multimode source is an ion source for LC/MS that can operate as an APCI, ESI or simultaneous APCI/ESI source. See Figure 26. The source is intended to operate at normal chromatographic flow rates 50 to 2000 µL /min. It is capable of fast reversing pos/neg switching. The multimode source can be run in three different modes of operation (MM-ES, MM-APCI or MM-ES+APCI).
Reference Benefits of a multimode source 3 • Permits analysis of fast-eluting peaks • Operation at flow rates used for high-throughput analysis without splitting • Operation at lower flow rates for higher-sensitivity analysis The design uses a single nebulizer through which the LC effluent flows. You do not need to switch back and forth between APCI and ESI modes. While this option will be available, the great value of the source is its ability to produce APCI and ESI generated ions simultaneously.
3 Reference Benefits of a multimode source Multimode source operational description Figure 27 Graphical representation of multimode source 1 Liquid enters the grounded nebulizer 2 A charged aerosol is made in the ESI Zone 3 The aerosol is dried by IR lamps 4 Neutral analytes and ESI charged analytes pass through the APCI Zone 5 ESI and APCI ions enter the capillary 46 Multimode Source for 6100 Series Single Quad LC/MS User Guide
Reference Benefits of a multimode source 3 MM-ES Zone Figure 28 Graphical representation of MM-ES zone 1 Liquid enters the grounded nebulizer.
3 Reference Benefits of a multimode source Infrared Drying Figure 29 Graphical representation of infrared drying 1 The charged aerosol passes the reversing electrode and enters the thermal container.
Reference Benefits of a multimode source 3 MM-APCI Zone Figure 30 Graphical representation of MM-APCI zone 1 Neutral analytes and MM-ES charged analytes pass through the MM-APCI Zone. 2 A corona is formed between the APCI corona needle and the pin. 3 Neutral analytes in or very near this gap are ionized. 4 MM-ES and MM-APCI ions are merged and follow the electric field into the mass spectrometer.
3 Reference Benefits of a multimode source Multimode source side view Figure 31 50 Graphical representation of a side view of the multimode source Multimode Source for 6100 Series Single Quad LC/MS User Guide
Reference Benefits of a multimode source 3 Multimode source top down view Figure 32 Graphical representation of a top view of the multimode source Multimode Source for 6100 Series Single Quad LC/MS User Guide 51
3 Reference Mobile phase considerations when using the multimode source Mobile phase considerations when using the multimode source Flow rate In general, once the analyte response is optimized, the flow rate or mobile phase composition may be changed without having to re-optimize the vaporizer temperature or drying gas temperature. The IR emitters have a feedback control that is provided by the vapor temperature sensor.
Reference Mobile phase considerations when using the multimode source 3 • In negative APCI mode, the use of acetonitrile has also been linked with a reduction in response of the analytes. For electrolytes added to the mobile phase used for mixed mode operation, use care in selecting the anion as it may affect the response in negative APCI mode.
3 Reference Flowchart summarizing parameter choices for different modes Flowchart summarizing parameter choices for different modes 54 Multimode Source for 6100 Series Single Quad LC/MS User Guide
Reference Source comparison in positive ion mode 3 Source comparison in positive ion mode Using the multimode source in MM-ES+APCI mode with positive polarity, you can acquire a single data file containing the two compounds, indol and crystal violet.
3 Reference Source comparison in negative ion mode Source comparison in negative ion mode Using the multimode source in MM-ES+APCI mode with negative polarity, you can acquire both hexanesulfonic acid and dinitrobenzotrifluride in the same file. Neither ESI nor APCI can acquire both of these compounds.
Reference Single analysis in MM-ES+APCI mode with fast switching 3 Single analysis in MM-ES+APCI mode with fast switching Using the multimode source in MM-ES+APCI mode, you can use fast switching to acquire all four peaks during the same run.
3 Reference Guidelines Guidelines Guidelines for multimode source settings in MM-ES mode NOTE Click Instrument > MSD Spray Chamber to set the Method Spray Chamber to MM-ES+APCI before you edit the Set Up MSD Signals dialog box. Nebulizer pressure Always set to 60 psi. Drying gas flow Always set to 5 L/min (IR does the drying). Drying gas temperature Depends on the vaporizer temperature. A good starting temperature is 250°C.
Reference Guidelines for multimode source settings in MM-APCI mode 3 Guidelines for multimode source settings in MM-APCI mode NOTE Click Instrument > MSD Spray Chamber to set the Method Spray Chamber to MM-ES+APCI before you edit the Set Up MSD Signals dialog box. Nebulizer pressure Start at 20 psi. The signal decreases with higher nebulizer pressure. Drying gas flow Always set to 5 L/min (IR does the drying) Drying gas temperature Just set to 350°C.
3 Reference Guidelines for multimode source settings in MM-ES+APCI mode Guidelines for multimode source settings in MM-ES+APCI mode NOTE Click Instrument > MSD Spray Chamber to set the Method Spray Chamber to MM-ES+APCI before you edit the Set Up MSD Signals dialog box. Drying gas flow Always set to 5 L/min (IR does the drying) Nebulizer pressure Start at 40 psi. You will use the nebulizer pressure and the corona current to balance ESI and APCI. A higher nebulizer pressure results in more ESI.
Reference Guidelines for positive/negative polarity switching in mixed mode 3 Guidelines for positive/negative polarity switching in mixed mode NOTE Click Instrument > MSD Spray Chamber to set the Method Spray Chamber to MM-ES+APCI before you edit the Set Up MSD Signals dialog box. The following settings are a good starting point when you are doing positive/negative polarity switching in MM-ES+APCI mode. Drying gas flow Always set to 5 L/min (IR does the drying). Nebulizer pressure Start at 40 psi.
3 Reference Guidelines for positive/negative polarity switching in mixed mode NOTE 62 The above set of parameters is provided as a starting point and should yield good response for many analytes. To obtain a specific response, i.e., high sensitivity detection of a compound or class of compounds, or a balanced response to a wide variety of compound classes, these parameters and the LC mobile phase composition should be examined further using traditional techniques for optimizing a method.
Reference Empirical Formulas for high resolution MS 3 Empirical Formulas for high resolution MS G2421A MM-ES Calibration Ions Positive Ion (m/z) Empirical Formula Betaine+H 118.086809 C5.H12.O2.N HP-0321+H 322.048699 C6.H19.O6.N3.P3 HP-0622+H 622.029499 C12.H19.O6.N3.P3.F12 HP-0922+H 922.010300 C18.H19.O6.N3.P3.F24 1307.969049 C25.H17.O6.N3.P3.F40 HP-1521+H 1521.971900 C30.H19.O6.N3.P3.F48 fragment 1807.937049 C35.H17.O6.N3.P3.F60 HP-2121+H 2121.933500 C42.H19.O6.N3.P3.
3 Reference G2422A MM-APCI Calibration Ions NOTE Fragment ion signals are progressively stronger at higher m/z by increasing CID energy. G2422A MM-APCI Calibration Ions Positive Ion (m/z) Purine+H 121.051421 C5.H5.N4 HP-0321+H 322.048699 C6.H19.O6.N3.P3 HP-0622+H 622.029499 C12.H19.O6.N3.P3.F12 HP-0921+H 922.010300 C18.H19.O6.N3.P3.F24 fragment 1307.969049 C25.H17.O6.N3.P3.F40 HP-1521+H 1521.971900 C30.H19.O6.N3.P3.F48 fragment 1807.937049 C35.H17.O6.N3.P3.F60 HP-2121+H 2121.
Reference Tuning Mix and Test Mix 3 Tuning Mix and Test Mix G2421A ES Tuning Mix (100mL) Item-Description Quantity es tune mix label 1 each plastic bottle 1 each acetonitrile 74.67 g hp-0321 <1 mg hp-0621 <1 mg hp-0921 <5 mg hp-1521 <5 mg hp-2121 <5 mg hp-2721 <10 mg hp-0585 <5 mg betaine <1 mg tfa ammonium salt 26.
3 Reference G2422A APCI Tuning Mix (100mL) G2422A APCI Tuning Mix (100mL) 66 Item-Description Quantity apci tune mix label 1 each plastic bottle 1 each acetonitrile 62.88 g methanol 11.87 g hp-0321 <1 mg hp-0621 <1 mg hp-0921 <5 mg hp-1521 <5 mg hp-2121 <5 mg purine <1 mg chloroform 1.
Reference Chemical structures in the multimode test mix 3 Chemical structures in the multimode test mix The following diagram shows the chemical structure of the four components in the multimode test mix.
3 68 Reference Chemical structures in the multimode test mix Multimode Source for 6100 Series Single Quad LC/MS User Guide
Index Index A I T APCI Tuning Mix, 66 autotune, 22 installation, 23 technical specifications, 43 L B LC/MSD sample tubing, 36 Bake out the LC/MSD, 21 M C chemical structure in text mix, 67 clean source daily, 16 weekly, 19 converting from ESI, APCI or APPI, 31 converting to ESI or APCI, 30 D diverter valve inlet filter, 36 E ES Tuning Mix, 65 ESI convert from, 31 convert to, 30 F method alternating ESI and APCI, 11 basic setup, 8 positive/negative mixed mode, 9 mobile phase, 52 multimode nebul
Index 70 Multimode Source for 6100 Series Single Quad LC/MS User Guide
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