Technical data

Multimode Source for 6400 Series Triple Quad LC/MS Set-Up Guide 23
Performance Verification 2
To review methods and run worklist
4 In the method, select channel A, the 75:25 methanol/water solution with 5
mM ammonium formate, as the LC solvent.
5 Click File > Load and then load the method MMI-ES Pos MS2 Scan.m.
6 In the method, select channel A, 75:25 methanol/water solution with 5mM
ammonium formate, as the LC solvent.
7 Place the vials into the LC autosampler.
• Position #1: An empty, uncapped vial
• Position #2: A vial containing the solvent used for dilution (this is the
solvent blank)
• Position #3: A vial containing the reserpine sample (500 fg/µl)
8 From the File menu click Load and then load the worklist: MMI-ES Pos
Reserpine Checkout.wkl.
The worklist is set up to do one injection of the solvent blank using the ESI
Pos MS2 Scan.m method in order to collect background ion data, then using
the ESI Pos MRM Reserpine Checkout.m method for the remaining runs, one
injection of the empty vial, five injections of the solvent blank, and five
injections of the reserpine sample.