Technical data

22 Multimode Source for 6400 Series Triple Quad LC/MS Set-Up Guide
2 Performance Verification
To review methods and run worklist
To review methods and run worklist
You use the multimode source in Positive MRM Mode.
1 Verify that the Collision Cell gas flow rate produces a high vacuum gauge
reading in the range of 2.7 to 3.3 x 10e-5 torr.
To view the high vacuum gauge reading, click the Cell tab in the Tune
Context and check that the high vacuum gauge reading is within range. If
not, see “To reset the Collision Cell gas flow rate” in the Triple Quad
Installation Guide.
2 Start the Data Acquisition program, change the Context to Tune, and start
an Autotune.
After the autotune has completed, you may need to wait up to 30 minutes to
allow for the calibrant solution to be pumped out of the Triple Quad. This
minimizes any background signal attributable to the calibrant. In addition,
you can sonicate the nebulizer in a small graduated cylinder filled with
acetonitrile for 10 minutes.
3 Change the Context to Acquisition, click File > Load and then load the
method MMI-ES Pos MRM Reserpine Checkout.m. The checkout method
includes the following acquisition parameters:
• 2 µL injection
• isocratic from channel A at 0.4 mL/min.
• 2.5 minutes run time
• 250°C drying gas temperature
• 5 L/minute drying gas flow (can be optimized, typical 10 to 13 L/min)
• 60 psi nebulizer pressure (can be optimized, typical 35 to 40 psi)
• 2000 V capillary voltage
• 250°C Vaporizer temperature
• 2000 V charge voltage
• 37 V collision cell energy (can be optimized, typical between 37 to 41 V)
• 240 V fragmentor voltage (can be optimized, typical between 140 to 250
V)
• 700 V Delta EMV (can be optimized, typical between 400 to 800 V)
• MS1 Resolution set to Wide and MS2 Resolution set to Unit