Agilent G1978B Multimode Source for 6410 Triple Quad LC/MS Set-Up Guide Agilent Technologies
Notices © Agilent Technologies, Inc. 2008 Warranty No part of this manual may be reproduced in any form or by any means (including electronic storage and retrieval or translation into a foreign language) without prior agreement and written consent from Agilent Technologies, Inc. as governed by United States and international copyright laws. The material contained in this document is provided “as is,” and is subject to being changed, without notice, in future editions.
In This Guide This guide explains how to install, maintain and troubleshoot your multimode ion source. 1 Installation This chapter tells you how to install the multimode source. 2 Performance Verification This chapter describes how to set up methods and autotune the source.
Multimode Source for 6400 Series Triple Quad LC/MS Set-Up Guide
Content 1 Installation 7 Installation 8 Step 1. Prepare to install 8 Step 2.
Contents 6 Multimode Source for 6400 Series Triple Quad LC/MS Set-Up Guide
Agilent G1978B Multimode Source for 6410 Triple Quad LC/MS Set-Up Guide 1 Installation Installation 8 Step 1. Prepare to install 8 Step 2. Install the HV control PCA and cables 9 Changing Sources 13 To remove the multimode source 13 To convert from multimode to ESI or APCI 14 To convert from ESI or APCI to the multimode source 15 This chapter contains instructions to install the multimode source on a 6400 Series Triple Quad LC/MS system, and also to remove and replace the source.
1 Installation Installation Installation Step 1. Prepare to install The Multimode Enablement Kit, G1978-60451, is shipped with the multimode source. This kit needs to be installed before the multimode source is used. Note that the multimode source and its accessories are to be installed by an Agilent Customer Engineer.
Installation Step 2. Install the HV control PCA and cables Figure 2 1 From left to right: G1946-80058, G1960-60802 and G1960-60856 Step 2. Install the HV control PCA and cables 1 Turn off the system power and remove the system power cord. The power cord should be kept intact if the vacuum control switch box is used. The switch box is intended to keep the vacuum on while a service engineer works on the electronics. The switch box is for service engineer use only.
1 Installation Step 2. Install the HV control PCA and cables Figure 4 Connecting the valve PCA to the multimode HV power supply. 6 Install the APCI HV power supply. The APCI HV power supply is located at the end of the AUX Module. 7 Connect ribbon cable between the valve PCA and Vcap/Vchamber power supply. Figure 5 Connecting the valve PCA to the Vcap/Vchamber power supply. 8 Connect the Vcap and Vchamber cables to the Vcap/Vchamber power supply.
Installation Step 2. Install the HV control PCA and cables Figure 6 1 Connecting the Vcap and Vchamber cables to the power supply. 9 Connect the long ribbon cable, p/n G1960-60802, from the APCI HV power supply to the valve PCA. Figure 7 Connecting the APCI HV power supply to the valve PCA. 10 Insert one end of the APCI Needle Interlock cable, G1960-60856, through the slot at the front of the system and then plug it to the APCI HV connector.
1 Installation Step 2. Install the HV control PCA and cables 11 Insert the cable, G1960-60858, to the top slot and attach it to the chassis. Plug the other two ends into the multimode HV PCA. Figure 9 Connecting the HV PCA to the chassis. 12 Close the AUX Module cover and reconnect all cables. 13 Install the multimode source onto the system and connect all connectors. Figure 10 Installing the multimode source (left) and connecting all connectors. 14 Put back the side, top, front and CDS cover.
Installation Changing Sources 1 Changing Sources To remove the multimode source Do the following steps to remove the multimode source. 1 Turn off the multimode source temperatures and flows: a Change the Context view to Acquisition. b Click the MS QQQ tab. c Turn off all voltages and temperatures in the Source tab. WA R N I N G Do not touch the multimode source or the capillary cap. They may be very hot. Let the parts cool before you handle them.
1 Installation To convert from multimode to ESI or APCI To convert from multimode to ESI or APCI WA R N I N G Never touch the source surfaces, especially when you analyze toxic substances or when you use toxic solvents. The source has several sharp pieces which can pierce your skin including the APCI corona needle, vaporizer sensor and counter current electrode. 1 Unscrew and remove the multimode spray shield with the field shaping electrodes.
Installation To convert from ESI or APCI to the multimode source 1 To convert from ESI or APCI to the multimode source CAUTION If you are installing this source on this instrument for the first time, follow the steps in “Installation” on page 7. 1 Turn off the multimode source temperatures and flows: a Change the Context view to Acquisition. b Click the MS QQQ tab. c Turn off all voltages and temperatures in the Source tab. 2 Wait for the source to cool (until temperatures are at least below 100°C).
1 Installation To convert from ESI or APCI to the multimode source Standard spray shield Capillary cap Figure 11 Standard spray shield and capillary cap for ESI or APCI 8 Remove the capillary cap. If needed, moisten a clean cloth with isopropyl alcohol and wipe the capillary cap. See Figure 12. Capillary cap Figure 12 Spray shield removed. 9 Place the capillary cap back on the capillary. 10 Install the new spray shield with field shaping electrodes. See Figure 13.
Installation To convert from ESI or APCI to the multimode source Figure 13 1 Multimode spray shield 11 Screw the multimode spray shield into the holder for the spray shield. See Figure 14. Field shaping electrode 9 o'clock position Field shaping electrode 6 o'clock position Figure 14 NOTE Multimode spray shield installed The field shaping electrodes should be in the nine o’clock and the six o’clock positions.
1 Installation To convert from ESI or APCI to the multimode source 12 Remove the shipping cover from the multimode source spray chamber. Figure 15 Multimode Spray Chamber 13 Install the spray chamber on the spray chamber mount. I-Button Figure 16 Multimode source with I-Button 14 Install the nebulizer on the multimode source spray chamber.
Installation To convert from ESI or APCI to the multimode source Figure 17 1 No nebulizer on top of the multimode source 15 Connect the 1/8-inch nebulizer gas tubing from the LC/MS mainframe to the nebulizer gas fitting. See Figure 18.
1 Installation To convert from ESI or APCI to the multimode source 16 Connect the LC/MS sample tubing to the LC/MS diverter valve inlet filter. WA R N I N G The LC/MS Liquid Chromatograph diverter valve is an integral part of the G1978B safety system. The LC mobile phase flow must always be connected to the diverter valve inlet filter. Never bypass the diverter valve and connect directly to the nebulizer.
Agilent G1978B Multimode Source for 6410 Triple Quad LC/MS Set-Up Guide 2 Performance Verification To review methods and run worklist 22 To do sensitivity verification 24 To autotune with the multimode source 27 To set up a method to use the multimode source 28 This chapter contains the steps necessary to complete the hardware setup and verify the performance specifications of the installed system.
2 Performance Verification To review methods and run worklist To review methods and run worklist You use the multimode source in Positive MRM Mode. 1 Verify that the Collision Cell gas flow rate produces a high vacuum gauge reading in the range of 2.7 to 3.3 x 10e-5 torr. To view the high vacuum gauge reading, click the Cell tab in the Tune Context and check that the high vacuum gauge reading is within range. If not, see “To reset the Collision Cell gas flow rate” in the Triple Quad Installation Guide.
Performance Verification To review methods and run worklist 2 4 In the method, select channel A, the 75:25 methanol/water solution with 5 mM ammonium formate, as the LC solvent. 5 Click File > Load and then load the method MMI-ES Pos MS2 Scan.m. 6 In the method, select channel A, 75:25 methanol/water solution with 5mM ammonium formate, as the LC solvent. 7 Place the vials into the LC autosampler.
2 Performance Verification To do sensitivity verification To do sensitivity verification Figure 19 Reserpine checkout worklist 1 Review the worklist to be sure that the method and data paths are correct, and that the data file names given in the worklist are unique and have not already been acquired. 2 Run the worklist. 3 When the worklist is finished, calculate signal-to-noise for each injection: a Load each solvent blank and reserpine sample data file into the Qualitative Analysis program.
Performance Verification To do sensitivity verification Figure 20 2 Extract Chromatogram dialog box c Integrate each reserpine peak, and click Calculate Signal to Noise. d Calculate the signal-to-noise using Height. e Under Noise Measurement, for Noise definition, click RMS and select X 3. For Noise regions, type 1.00 - 1.45. (Make sure that the noise region does not include the reserpine peak. If the reserpine peak elutes within the default noise region, shift the noise region such that it is 0.
2 Performance Verification To do sensitivity verification 4 Print each signal-to-noise calculation report for each solvent blank and reserpine injection. Include the chromatogram in the printout. 5 Open the Excel spreadsheet D:\MassHunter\Support\Checkout\Sensitivity Checkout Report.xls. Fill in the values to calculate the average signal to noise and save the spreadsheet. The average signal-to-noise must be greater than 50:1. 6 Print the Excel signal-to-noise report.
Performance Verification To autotune with the multimode source 2 To autotune with the multimode source 1 Remove the G1948B Electrospray source and install the G1978B multimode source. 2 Pour the Electrospray or APCI calibrant back into its original bottle or another suitable container, rinse the calibrant bottle with acetonitrile, pour the MMI-L Low Concentration Tuning Mix (G1969-85020) calibrant into the calibrant bottle, and attach the calibrant bottle back onto the CDS.
2 Performance Verification To set up a method to use the multimode source To set up a method to use the multimode source 1 In the MassHunter software, change the Context to Acquisition. 2 In the MS QQQ tab, set Ion source to MMI (see Figure 22 on page 29). 3 In the Time Segments table, chose an ionization mode from the Ion Mode list.
Performance Verification To set up a method to use the multimode source Figure 22 2 Multimode acquisition settings WA R N I N G The LC/MS diverter valve is an integral part of the G1978B safety system. The LC mobile phase flow must always be connected to the diverter valve inlet filter. Never bypass the diverter valve and connect directly to the nebulizer.
2 30 Performance Verification To set up a method to use the multimode source Multimode Source for 6400 Series Triple Quad LC/MS Set-Up Guide
Index Index A autotune, 27 C converting from ESI or APCI, 15 converting to ESI or APCI, 14 D diverter valve inlet filter, 20 E ESI convert from, 15 convert to, 14 I installation, 7 M method basic setup, 28 multimode nebulizer, 19 P parts multimode spray shield, 17 S spray shield for multimode source, 17 Multimode Source for 6400 Series Triple Quad LC/MS Set-Up Guide 31
Index 32 Multimode Source for 6400 Series Triple Quad LC/MS Set-Up Guide
www.agilent.com In This Book This book contains installation, operation, maintenance and troubleshooting instruction for the Multimode Source for 6410 Triple Quad LC/MS. © Agilent Technologies, Inc.
