Technical data

Multimode Source for 6510 Q-TOF LC/MS Set-Up Guide 25
Set-Up 2
To check tuning with the multimode source
To check tuning with the multimode source
Autotune is currently only available for the G3251B Dual Electrospray source.
However, mass calibrations and manual optimization of mass resolution can
be done using the G1978B source. To calibrate mass accuracy, do these steps.
1 Run an Autotune with the G3251B Dual Electrospray source installed.
2 Remove the G3251B Dual Electrospray source and install the G1978B
multimode source.
3 Uninstall the Electrospray Calibrant Bottle B from the instrument. Cap the
calibrant bottle with one of the supplied bottle caps (p/n 9300-2575).
4 Rinse one of the extra calibrant bottles (p/n 9300-2576) that was supplied
as part of the Q-TOF Shipping Kit (p/n G2581-60170) with high purity
acetonitrile. Pour the contents of the MMI-L Low Concentration Tuning Mix
(p/n G1969-85020) into the rinsed calibrant bottle. Install the calibrant
bottle on the Q-TOF mainframe in the bottle B location.
5 Set the Context view to Tune in the MassHunter Workstation program.
a Load the most recently used autotune file. Change the source type
Multimode.
b Click the Mass TOF Calibration tab and do a mass calibration.
c Adjust the lens voltages and other tune parameters as required to
optimize the mass resolution of the instrument. If changes are made to
the Mid Mirror, a mass calibration will have to be done again.
d Verify that you have sufficient abundance for the tune peaks, that the
tune peak at 2122 has greater than 10,000 resolution, and that all mass
assignments are with 2 ppm after a mass calibration has been done.
6 Save the tune file and close the tune context.