User`s guide

Temperature Control 13
Agilent 5500 SPM User’s Guide 207
Figure 141 Gravity-fed water cooling system for Peltier sample plate.
Imaging
1 Set up the microscope for typical operation. As mentioned above,
uncoated silicon probes are highly recommended.
2 Mount the sample on the sample plate.
3 Set the Range control on the current booster to its minimum setting
(fully counterclockwise).
4 Set the current booster to 1X.
5 Turn on the Lakeshore controller and current booster.
6 On the Lakeshore controller’s front panel, press Auto Tune, then
press the Up or Down arrow buttons until the display read
Tune:Manual. Press Enter.
7 Set the Proportional, Integral and Differential gains. Typical
values are 12, 12 and 5 respectively.
8 Press Setpoint and enter a value slightly lower than room
temperature 23 C. Wait for the setpoint temperature to stabilize.
9 Set the Ramp Rate to no more than 10 degrees per minute (5
degrees/minute is a typical ramp rate).
10 Turn the Range control on the current booster to maximum (fully
clockwise).
NOTE
The Lakeshore controller will attempt to adjust the temperature to the last
selected temperature as quickly as possible. Depending on the sample,
and the last temperature setting, this can be detrimental to the plate
and/or sample. Setting the temperature to ambient avoids this problem.