User`s guide

Additional Imaging Modes 7
Agilent 5500 SPM User’s Guide 121
6
In PicoView, choose Mode > CSAFM.
7 In the Servo window enter the Bias Voltage. Typical values are
50-200 millivolts (0.05-0.200 V).
8 Using a voltmeter, check the potential between the working electrode
contact and ground (the exposed metal of the DB44 connector on the
microscope is a good ground point). The bias should be the same as
that entered in the Servo window. If it is not, you may need to adjust
the controller calibration (see the PicoView software user guide for
additional information).
9 Enter a Setpoint value that is slightly more positive than the current
Deflection reading (on the HEB front panel or PicoView’s Laser
Alignment window).
10 Enter the I and P gains for the z-servo, which will dictate how
quickly the system will adjust to changes in tip deflection. A typical
starting value is 10 % for both gains.
11 In the Realtime Images window choose to display images for
CSAFM/Aux BNC, Deflection and Topography.
12 In the Scan and Motor window’s Scan tab, enter:
a Scan Speed of 1-2 ln/s.
b Resolution of 256.
c Scan Size (in microns).
d X Offset and/or Y Offset values to set the location of the scan
center.
13 Press the Close switch on the HEB to raise the sample until the tip is
close to, but not touching, the sample.
NOTE
The sample plate cable can transfer low levels of vibration to the sample.
During very high resolution imaging this can affect resolution. We
recommend first plugging the sample plate cable to the 3-wire umbilical
included with the sample plate. The umbilical should then be plugged in
to the microscope base. The umbilical’s individual wires tend to reduce
the transfer of vibration.