User guide

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Fragment Library Preparation Using the AB Library Builder
™
System: 5500 Series SOLiD
™
Systems User Guide
Glossary
P1-T Adaptor
A T-tailed double-stranded oligonucleotide containing the P1 sequence that is ligated
to A-tailed DNA segments during library construction; the result is that the P1
sequence is attached to the 5' end of the template strand.
Standard Adaptor
During fragment library preparation, the double-stranded oligonucleotide that is
ligated to the genomic DNA fragment such that the internal adaptor, barcode sequence
BC-001, and the P2 Adaptor are at the 3' end of the sequencing template.
tag
There are two uses for this term.
• A length of DNA or cDNA to be sequenced; especially, a relatively short stretch of
DNA or cDNA that is used to infer information about the longer native molecule
from which it is derived, such as in SAGE™ analysis and mate-pair library
sequencing.
• Sequencing data from a single bead with a single primer set; sometimes used
interchangeably with read.
templated bead
preparation
Process of covalently attaching and clonally amplifying template strands to beads by
emulsion PCR, enriching the beads to remove beads without template, then modifying
the 3' end of the template on the beads to prepare for bead deposition and sequencing