User guide

Fragment Library Preparation Using the AB Library Builder

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System: 5500 Series SOLiD

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Systems User Guide

AppendixB Supplemental Procedures

(Optional) Size-select and pool libraries

Move the axes to the original positions and/or return the tip to the origin as

follows:

Note: When the run is interrupted, the axes and tip do not automatically return to

the original positions.

• If the tips need to be returned to the holders – Press 2 (Return Tip) to return

the tips to the tip holders and move all axes to the original position:

• If the tips do not need to be returned to the holders –

–Press 1 (ORG) to go to the ORG screen:

– Move each individual axis to the origin by pressing 1, 2, 3, 4,

respectively, or press 0 to return all axes to the origin.

5. Press ESC to return to Main menu:

You are now ready to set up for a new run.

(Optional ) Size-select and pool libraries

Size-select the

barcoded libraries

Prepare the SOLiD Library Size Selection gel

The DNA is run on a SOLiD

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Library Size Selection gel. The correctly sized ligation

products (~240–270 bp) are electrophoresed to the collection wells of the SOLiD

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Size

Selection Gel. The eluates in each collection well are pooled.

1. Remove a SOLiD

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Library Size Selection gel from its package. Remove the combs

from top sample-loading wells and middle collection wells.

2. Set the SOLiD Library Size Selection Gel on the E-Gel

iBase

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system linked with

the E-Gel

Safe Imager

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Real-Time Transilluminator.