User guide

47
Fragment Library Preparation Using the AB Library Builder
™
System: 5500 Series SOLiD
™
Systems User Guide
B
APPENDIXB
Supplemental Procedures
This appendix covers:
■ Load and unload Covaris
®
microTUBE vials from the Covaris
®
microTUBE
holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
■ AB Library Builder
™
System operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
■ (Optional) Size-select and pool libraries . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
■ Quantitate the DNA with the NanoDrop
®
ND-1000 Spectrophotometer . . . . . . 55
Load and unload Covaris
®
microTUBE vials from the Covaris
®
microTUBE holder
Load Covaris
®
microTUBE vials
1. Use a thumb to push the stainless steel plunger up into the body of the
microTUBE holder.
2. Place the body of the microTUBE against the two amber plastic prongs with the
cap of the microTUBE positioned above the prongs.
3. Use a finger to press against the middle of the glass tube (not against the cap).
With a single motion, push the tube between the prongs to position the tube: