User guide

45
Fragment Library Preparation Using the AB Library Builder
™
System: 5500 Series SOLiD
™
Systems User Guide
AppendixA Ordering Information
Required consumables
A
Required consumables
AB Library Builder
™
D-Rings 4465602
AB Library Builder
™
Plastics Module 4465605
Agilent DNA 1000 Kit Agilent Technologies
5067-1504
† Applied Biosystems has validated this protocol using this specific material.
Substitution may adversely affect system performance.
Product name
†
Vendor
Item
†
Source
1✕ Low TE Buffer Applied Biosystems
4389764
Nuclease-free Water, 1 L Applied Biosystems
AM9932
MicroAmp
®
Optical 8-Tube Strip, 0.2 mL Applied Biosystems
4316567
Invitrogen Qubit
™
dsDNA HS Assay Kit
or
Invitrogen Qubit
™
dsDNA BR Assay Kit
or
Invitrogen Quant-iT
™
PicoGreen
®
dsDNA
Assay Kit
Invitrogen
Q32851 or Q32854
Invitrogen
Q32850 or Q32853
Invitrogen
P7589
Agencourt AMPure
®
XP:
5mL Kit
or
60 mL Kit
or
450 mL Kit
Beckman Coulter
Genomics
A63880
or
A63881
or
A63882
Covaris
®
microTUBEs Covaris
520045
Ethanol, absolute Sigma-Aldrich
E7023