User guide

41
Fragment Library Preparation Using the AB Library Builder
™
System: 5500 Series SOLiD
™
Systems User Guide
AppendixA Ordering Information
Required Applied Biosystems reagent kits for automated liquid-handling systems
A
Required Applied Biosystems reagent kits for automated
liquid-handling systems
Note: Customers who have access to an automated liquid-handling system such as the
Beckman Coulter Biomek
®
FX
p
and Tecan Freedom EVO
®
instruments, can choose
from the kits below:
5500 SOLiD
™
Fragment Library Barcode
Adaptors 65–80
(4464409)
‡
Barcode adaptors
T-065–T-080
5500 SOLiD
™
Fragment Library Barcode
Adaptors 81–96
(44644140)
‡
Barcode adaptors
T-081–T-096
† Applied Biosystems has validated this protocol using this specific material. Substitution
may adversely affect system performance.
Item
†
Source
Item (part no.)
†
† Applied Biosystems has validated this protocol using this specific material. Substitution
may adversely affect system performance.
Components
5500 SOLiD
™
Fragment 48 Library Core Kit
(4464415)
• 5500 SOLiD
™
48
Fragment Library
Enzyme Module
• 5500 SOLiD
™
48
Fragment Library
Amplification Module
5500 SOLiD
™
48 Fragment Library Enzyme
Module
(4464416)
•10mM dNTP
• End Polishing E1
• End Polishing E2
•5✕ Reaction Buffer
•A-tailing Enzyme I
• T4 DNA Ligase, 5 U/µL
•10mM dATP
•Shear Buffer
5500 SOLiD
™
48 Fragment Library
Amplification Module
(4464417)
Platinum
®
PCR
Amplification Mix