User guide
28
Fragment Library Preparation Using the AB Library Builder
™
System: 5500 Series SOLiD
™
Systems User Guide
Chapter3 Build the library
Set up for a new run
b. Remove the elution tubes. Confirm that they are properly labeled, then cap
the elution tubes containing the library in 100 µL.
c. If the library has a brown tint, place each tube in a DynaMag
™
-2 Magnetic
Rack for at least 1 minute until the solution is clear of brown tint when
viewed at an angle; then transfer the supernatant to a new tube.
d. Remove the tip and tube rack and cartridge rack.
e. Properly dispose of the used reagent cartridges, tips, and tubes.
f. Close the instrument door.
g. Clean the tip and tube rack as needed.
Note: No cooling period is required between runs.
STOPPING POINT Store the DNA in a supplied Sample Tubes at 4°C for short-term
storage or at –20°C for long-term storage, or proceed directly to “Nick-Translate
the Library with Optional Amplification” on page 29.
Set up for a new run
WARNING! Do not clean the instrument with acids, or bases (such as bleach).
Acids and bases can react with the guanidine thiocyanate in the lysis buffer and
generate toxic gas.
1. Follow the set-up procedures for a new run (see “Set up the AB Library Builder
™
System for size-selected or express fragment library preparation” on page 17).
Note: To set up for a new run using the same protocol card, leave the instrument
on. To set up for a new run with a different protocol card, power off the
instrument, then change the protocol card (see “Insert or change the protocol card
and power ON the instrument” on page 18).
2. Start the run (see “Start the run” on page 27).