User guide
20
Fragment Library Preparation Using the AB Library Builder
™
System: 5500 Series SOLiD
™
Systems User Guide
Chapter2 Prepare to Build the Library
Set up the AB Library Builder
™
System for size-selected or express fragment library preparation
Load the cartridge rack
1.
Remove up to 13 cartridges from the kit box.
IMPORTANT!
One cartridge is required per sample. Use only AB Library Builder™ Cartridges.
IMPORTANT!
Do not switch the supplied pre-filled reagents with any other buffers, because the
protocols are specifically optimized with the reagents supplied with the kit.
2. Thaw the cartridges at room temperature or on ice for ≤ 2 hours or until
completely thawed.
IMPORTANT! Avoid leaving the cartridges at room temperature for longer than
necessary to completely thaw them. Avoid repeated freeze-thawings of unused
cartridges.
IMPORTANT! Before loading the cartridges into the cartridge rack, ensure that the
cartridges are completely thawed, particularly reagents in cartridge wells 2 and 3.
3. Gently tap each cartridge on the laboratory bench until any liquid droplets that
might be underneath the foil seal are deposited into the bottom of the well.