User`s guide

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Preparing Samples and Chips for Electrophoretic Assays
Before you can fill a chip, you have to prepare the samples. To find out how to prepare the
samples, refer to the various Reagent Kit Guides available for each LabChip kit. Please
refer to these documents for further information and analytical specifications.
In general, preparing an electrophoretic assay involves the following steps:
• Check that you have everything listed in the appropriate Reagent Kit Guide.
Be aware that there can be small but important differences between the different
assays even for the same type of molecules (for example, between DNA 1000 and DNA
7500 assays).
• Make sure you are familiar with the essential measurement practices (see below).
• Before running the first RNA assay: decontaminate the electrodes.
• Prepare all the reagent mixtures (for example, the gel-dye mix).
• Load the gel-dye mix using the priming station.
• Load the DNA/RNA marker solution.
• Load the chip with ladder, samples and buffer (depending on assay).