User`s guide

Contents ▲ 60 ▼ Index
Preparing and Running an Electrophoretic Assay
An electrophoretic chip run requires the following steps:
1. Set up and switch on the Agilent 2100 bioanalyzer.
Refer to “Switching Between Electrophoretic and Flow Cytometric Assays” on page 47.
2. Start the 2100 expert software.
Details are given in “Starting 2100 Expert” on page 32.
3. Select an electrophoretic assay.
See “Selecting an Electrophoretic Assay for a Chip Run” on page 62.
4. Prepare chip and samples.
Refer to “Preparing Samples and Chips for Electrophoretic Assays” on page 66 and to
the appropriate Application Note and Reagent Kit Guide.
5. Load the chip into the bioanalyzer.
For details refer to “Loading the Electrophoresis Chip into the Bioanalyzer” on page 70.
6. Start the chip run.
This is described in “Running an Electrophoretic Assay” on page 72.
When the chip run has finished, you can:
• Have a first look at the results (see “Displaying the Measurement Results
(Electrophoresis)” on page 82).
• Document the chip run (see “Entering Chip, Sample, and Study Information” on
page 80).