User`s guide

Contents ▲ 593 ▼ Index
R
Raw data backup file
See PCK file.
S
Serial port
The serial ports (COM ports) are used to connect your computer with the Agilent 2100
bioanalyzer. The number of available ports depends on the computer you use.
Slope Threshold
The Slope Threshold setpoint represents the amount of change in fluorescence units
over time required to indicate that a peak has occurred. Changing this setpoint may
cause certain peaks that were previously detected to be ignored. The Slope Threshold
setting is one of the user-definable parameters in the setpoint explorer.
Standard Curve
The standard curve is obtained by plotting the size of the ladder peaks vs. time using a
point-to-point fit. For each sample peak, the center time is interpolated from the Standard
Curve to determine the peak size in base pairs.
Start Point
The peak find algorithm walks the data from time zero looking for a slope greater than the
Slope Threshold. This is considered to be the start point of a peak.
With RNA assays, individual peak start times can be moved manually by dragging the
diamond-shaped start points shown in the single view.