User`s guide

Contents ▲ 580 ▼ Index
NOTE
With RNA assays, you can move the lines marking the start and end points for data
analysis (shown by the long-dashed vertical green lines) which will adjust the entire
baseline for calculation of the area of the total sample.
Baseline Plateau
This setpoint (found in the setpoint explorer) rejects brief, low slope areas such as at
peaks and between non-baseline-resolved peaks. The signal is recognized to be at
baseline whenever the slope of the data is less than the Slope Threshold setpoint (either
positive or negative) for longer than the time set for the Baseline Plateau.
BMP file
BMP is the standard Windows image format. The BMP format supports RGB,
indexed-color, grayscale, and bitmap color modes.
Bubble
If the tip of a pipette is not positioned all the way to the bottom of a well, bubbles can
result (and sometimes bubbles happen even when you are very careful). The vortexing
step that occurs after samples are loaded into the chip is designed to rid the wells of
bubbles and is usually very effective.
If a large bubble is seen at the bottom of a well, remove the sample from the well, pipette
it back in, and continue with the loading procedure.