User`s guide

Contents ▲ 169 ▼ Index
Preparing and Running a Flow Cytometric Assay
A flow cytometric chip run requires the following steps:
1. Set up and switch on the Agilent 2100 bioanalyzer.
Refer to “Switching Between Electrophoretic and Flow Cytometric Assays” on page 47.
2. Start the 2100 expert software.
Details are given in “Starting 2100 Expert” on page 32.
3. Select a flow cytometric assay.
See “Selecting a Flow Cytometric Assay for a Chip Run” on page 171.
4. Prepare chip and samples.
Refer to “Preparing Samples and Chips for Flow Cytometric Assays” on page 176 and
to the appropriate Application Note and Reagent Kit Guide.
5. Load the chip into the bioanalyzer.
For details refer to “Loading the Cell Chip into the Bioanalyzer” on page 180.
6. Start the chip run.
This is described in “Running a Flow Cytometric Assay” on page 182.
When the chip run has finished, you can:
• Have a first look at the results (see “Displaying the Measurement Results (Flow
Cytometry)” on page 192).
• Document the chip run (see “Entering Chip, Sample, and Study Information” on
page 190).