User`s guide

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Setting the Baseline for Calculation of RNA Concentration
At low signal-to-noise ratios, the baseline that defines the area used for calculating the
concentration of RNA assays is highly dependent on the settings for the Start and End
Time. You can adjust the Start and End Times manually (thereby adjusting the baseline)
to ensure a good result even at very low signal-to-noise ratios. Choose a single sample.
Two vertical green long-dashed lines indicating the setpoints for the Start and End Times
(with the baseline drawn between them) are displayed in the window.
Move the cursor over the long-dashed line on the left (Start Time setting) and drag the
line to the desired position. Do the same with the long-dashed line on the right (End Time
setting) until you have a flat baseline.
NOTE
Changing the start and end times will change the calculated RNA concentration. It
might be convenient to pause the automatic analysis (Electropherogram > Pause
Automatic Analysis) until all changes are done.