User`s guide

Contents ▲ 104 ▼ Index
7. The standard curve, in conjunction with the markers, is used to calculate protein sizes
for each sample from the migration times measured.
8. To calculate the concentration of the individual proteins in all sample wells, the upper
marker, in conjunction with an assay-specific concentration calibration curve, is applied
to the individual sample peaks in all sample wells.
NOTE
The software allows you to define upper and lower markers yourself. A change in the
selection of the markers will lead to quantitative changes in the calibration procedure,
however, and therefore in the entire data evaluation.