Troubleshooting guide

Lab Exercise: Integration

Using Manual Events

Manual integration allows you to integrate specific peaks or groups of peaks with

complete control. For example, you may set the integration start and stop points

of any chromatographic peak yourself. This type of integration is convenient, but

may not be applicable from run to run. It also may not be as reproducible from

operator to operator.

1) Select the Manual Integration: Remove integrated Peak(s) from the

Integration Results tool. Note: Integration Events must be closed to manually

integrate.

2) Draw a rubber band box around one of the chromatographic peaks including

the integration start and stop tic marks. Notice that the peak is no longer

integrated.

3) Magnify an area of the chromatogram.

4) Now select the Manual Integration: Draw Peak Baselines(s) and integrate tool

or select the Draw Baseline selection from the Integration menu. Draw in

the baseline using the left mouse button. The Area is displayed to the side

after the integration is accomplished.

Other manual integration events include: Negative Peaks, Tangent Skim, Split

Peak, and All Valleys. Practice with these manual events.

Copying Manual Integration Events to a Method

The software allows you to copy the integration events you defined using manual

integration to the currently loaded method using the Copy Manual Events to

Method item from the Integration menu. These manual events are then stored

separately in the method. They cannot be reviewed in the Integration Events

dialog box. When a signal is loaded they are only applied when the Apply

Manual Integration option is checked in the Integration Events or when you

select Apply Manual Events from method in the Integration menu. To see

how this task works, do the following.