Troubleshooting guide

Lab Exercise: Quantification

Setting up Qualifiers (Diode Array only)

A qualifier peak is used to confirm that the main peak within the retention time

window belongs to the expected compound. It is assumed that the peaks of one

compound have a constant response ratio at two different wavelengths. The

qualifier peak response is a percentage of the main peak response.

1) Change from Compound Details to Identification Details either in the drop

box or through the menus: Calibration, Calibration Table Options...,

Identification Details.

2) Identify the Pk Usage column. Note that one signal for the biphenyl peak is

designated as the Main peak.

3) Notice the other signal. Change the peak usage from Ignore to Qualifier for

one of the peaks. The expected ratio is shown with a +/- 20% window.

4) Set up a qualifier for the o-terphenyl compound as well.

5) Resave the method. Type in a comment for method history.

You have now set up two curves with qualifiers. In the next section you will test

your calibration table and print a simple report.