User manual

72 Agilent 1260 Infinity High Performance Autosampler User Manual

5 Optimizing Performance

How to Achieve High Throughput

The injection can be optimized for speed remembering that drawing the

sample too fast can reduce the reproducibility. Marginal gains are to be

made here as the sample volumes used tend towards the smaller end of

the range in any case. A significant portion of the injection time is the

time taken with the needle movements to and from the vial and into the

flush port. These manipulations can be performed while the previous

separation is running. This is known as "overlapped injection" and it can

be easily turned on from the autosampler setup screen in the control

software. The autosampler can be told to switch the flow through the

autosampler to bypass after the injection has been made and then after,

for example, 3 minutes into a 4 minutes run to start the process of

aspirating the next sample and preparing for injection. This can typically

save 0.5 to 1 minute per injection.