User manual

Agilent 1260 Infinity High Performance Autosampler User Manual 71
Optimizing Performance
5
How to Achieve Higher Injection Volumes
but it is very dependent on the chemistry of the analyte and mobile phase
as discussed above. In a gradient separation injection volumes of about
5 % of the column volume might be achieved whilst maintaining good
resolution and peak dispersion.
One way to achieve larger injections is to use a trapping column selected
by a switching valve to capture and concentrate the injection before
switching it, i.e. injecting it, onto an analytical column, see Figure 17 on
page 71. The valve can be conveniently located in the Thermostatted
Column Compartment.
Figure 17 Sample enrichment