User manual
68 Agilent 1260 Infinity High Performance Autosampler User Manual
5 Optimizing Performance
How to Configure the Optimum Delay Volume
Figure 16 Schematic of injection steps in 1260 Infinity Autosampler
When using ADVR it should be noted that the gradient has already started
at the pump at the instant of injection. The question should be asked
whether the gradient has already reached the autosampler, in which case
a small step in the gradient will result. This happens when the delay
volume is less than the flush- out volume and is not necessarily a problem
but may be a factor to be considered in a method transfer. With a
flush- out factor of 5 and an injection volume of 10 µL, the autosampler
will allow 50 µL to pass through before switching to bypass which, with a
delay volume of 50 µL, means the gradient just reached the injection
valve. Smaller injection volumes will have no effect but for larger injection
volumes this will introduce a small step in the gradient. The flow rate in
use will also have an impact on the decision to use ADVR or not. At
0.2 mL/min the delay time saved is 21 seconds while at 1.0 mL/min it is
4 seconds.
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