User manual

50 Agilent 1260 Infinity High Performance Autosampler User Manual
4 Using the Module
Preparing the Autosampler
• Priming and Purging the System - When the solvents have been
exchanged or the system has been turned off for a certain time (for
example, overnight) oxygen will re- diffuse into the solvent channel.
Therefore priming and purging of the system is required before starting
an application.
Tabl e 4 Choice of Priming Solvents for Different Purposes
Activity Solvent Comments
After an installation Isopropanol Best solvent to flush air out of
the system
When switching between
reverse phase and normal
phase (both times)
Isopropanol Best solvent to flush air out of
the system
After an installation Ethanol or methanol Alternative to isopropanol
(second choice) if no
isopropanol is available
To clean the system when
using buffers
Bidistilled water Best solvent to re-dissolve
buffer crystals
After a solvent change Bidistilled water Best solvent to re-dissolve
buffer crystals