Agilent 1260 Infinity High Performance Autosampler User Manual Agilent Technologies
Notices © Agilent Technologies, Inc. 2010-2013, 2014 No part of this manual may be reproduced in any form or by any means (including electronic storage and retrieval or translation into a foreign language) without prior agreement and written consent from Agilent Technologies, Inc. as governed by United States and international copyright laws. Manual Part Number G1367-90014 Rev.
In This Guide In This Guide This manual covers the Agilent 1260 Infinity High Performance Autosampler (G1367E) 1 Introduction This chapter gives an introduction to the autosampler. 2 Site Requirements and Specifications This chapter provides information on environmental requirements, physical and performance specifications. 3 Installing the Autosampler This chapter provides information on unpacking, checking on completeness, stack considerations and installation of the autosampler.
In This Guide 8 Test Functions This chapter describes the tests for the module. 9 Maintenance This chapter describes the maintenance of the Autosampler 10 Parts for Maintenance This chapter provides information on parts material required for the module. 11 Identifying Cables This chapter provides information on cables used with the Agilent 1200 Infinity Series modules. 12 Hardware Information This chapter describes the autosampler in more detail on hardware and electronics.
Contents Contents 1 Introduction 9 Features 10 Overview of the Module 11 Autosampler Principle 13 System Overview 19 2 Site Requirements and Specifications 23 Site Requirements 24 Physical Specifications 27 Specifications 28 3 Installing the Autosampler 31 Unpacking the Autosampler 32 Optimizing the Stack Configuration 35 Installation Information on Leak and Waste Handling Installing the Autosampler 44 Flow Connections to the Autosampler 46 4 Using the Module 40 47 Leak and Waste Handling 48 Prep
Contents 6 Troubleshooting and Diagnostics 79 Overview of the Module’s Indicators and Test Functions Status Indicators 81 User Interfaces 83 Agilent Lab Advisor Software 84 7 Error Information 80 85 What are Error Messages 87 General Error Messages 88 Module Error Messages 93 8 Test Functions 105 Introduction 106 System Pressure Test 107 Sample Transport Self Alignment Maintenance Positions 112 Injector Steps 116 9 Maintenance 110 119 Introduction to Maintenance 120 Warnings and Cautions 121 Over
Contents 10 Parts for Maintenance 145 Overview of Maintenance Parts 146 Vial Trays 147 Recommended Plates and Closing Mats Recommended Vial Plates 149 Kits 150 Analytical Head Assembly 151 Injection Valve Assembly 152 Cover Parts 153 Leak System Parts 154 11 Identifying Cables 148 155 Cable Overview 156 Analog Cables 158 Remote Cables 160 BCD Cables 163 CAN/LAN Cables 165 External Contact Cable 166 Agilent Module to PC 167 Agilent 1200 Module to Printer 12 Hardware Information 168 169 Firmware Desc
Contents 14 Appendix 193 General Safety Information 194 Lithium Batteries Information 197 The Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC) 198 Radio Interference 199 Sound Emission 200 Use of Solvents 201 Agilent Technologies on Internet 202 8 Agilent 1260 Infinity High Performance Autosampler User Manual
Agilent 1260 Infinity High Performance Autosampler User Manual 1 Introduction Features 10 Overview of the Module Autosampler Principle 11 13 System Overview 19 Leak and Waste Handling 19 This chapter gives an introduction to the autosampler.
1 Introduction Features Features The 1260 Infinity High Performance Autosampler features an increased pressure range (up to 600 bar) enabling the use of today’s column technology (sub- two- micron narrow bore columns) with the Agilent 1260 Infinity Binary LC System .
1 Introduction Overview of the Module Overview of the Module The Autosampler transport mechanism uses an X- Z- theta robot to optimize the positioning of the sampling arm on the well plate. Once the sampling arm is positioned over the programmed sample position, the programmed sample volume is drawn by the metering device into the sampling needle. The sampling arm then moves to the injection position where the sample is flushed onto the column.
1 Introduction Overview of the Module Control of the vial/plate temperature in the thermostatted autosampler is achieved using an additional Agilent 1290 Infinity Series module; the Agilent 1290 Infinity Series thermostat for ALS/FC/Spotter. The thermostat contains Peltier- controlled heat- exchangers. A fan draws air from the area above the sample vial tray of the autosampler. It is then blown through the fins of the cooling/heating module. There it is cooled or heated according the temperature setting.
Introduction Autosampler Principle 1 Autosampler Principle The movements of the autosampler components during the sampling sequence are monitored continuously by the autosampler processor. The processor defines specific time windows and mechanical ranges for each movement. If a specific step of the sampling sequence is not completed successfully, an error message is generated. Solvent is bypassed from the autosampler by the injection valve during the sampling sequence.
1 Introduction Autosampler Principle Injection Sequence Before the start of the injection sequence, and during an analysis, the injection valve is in the mainpass position. In this position, the mobile phase flows through the autosampler metering device, sample loop, and needle, ensuring all parts in contact with sample are flushed during the run, thus minimizing carry- over.
Introduction Autosampler Principle 1 When the sample sequence begins, the valve unit switches to the bypass position. Solvent from the pump enters the valve unit at port 1, and flows directly to the column through port 6.
1 Introduction Autosampler Principle The standard injection starts with draw sample from vial. In order to do this the needle moves to the desired sample position and is lowered into the sample liquid in the sample to allow the metering device to draw up the desired volume by moving its plunger back a certain distance. The needle is then raised again and moved onto the seat to close the sample loop. In case of an injector program several steps are interspersed at this point.
Introduction Autosampler Principle Flush the Needle 1 Before injection and to reduce the carry- over for very sensitive analysis, the outside of the needle can be washed in a flush port located behind the injector port on the sampling unit. As soon as the needle is on the flush port a peristaltic pump delivers some solvent during a defined time to clean the outside of the needle. At the end of this process the needle returns to the injection port.
1 Introduction Autosampler Principle Inject-and-Run The final step is the inject- and- run step. The six- port valve is switched to the main- pass position, and directs the flow back through the sample loop, which now contains a certain amount of sample. The solvent flow transports the sample onto the column, and separation begins. This is the beginning of a run within an analysis. In this stage, all major performance- influencing hardware is flushed internally by the solvent flow.
Introduction System Overview 1 System Overview Leak and Waste Handling The 1200 Infinity Series has been designed for safe leak and waste handling. It is important that all security concepts are understood and instructions are carefully followed.
1 Introduction System Overview & 6 ' 7 8 ( ) * , + , Figure 6 20 Leak and waste handling concept (overview - typical stack configuration as an example) Agilent 1260 Infinity High Performance Autosampler User Manual
1 Introduction System Overview The solvent cabinet (1) is designed to store a maximum volume of 6 L solvent. The maximum volume for an individual bottle stored in the solvent cabinet should not exceed 2.5 L. For details, see the usage guideline for the Agilent 1200 Infinity Series Solvent Cabinets (a printed copy of the guideline has been shipped with the solvent cabinet, electronic copies are available on the Internet).
1 22 Introduction System Overview Agilent 1260 Infinity High Performance Autosampler User Manual
Agilent 1260 Infinity High Performance Autosampler User Manual 2 Site Requirements and Specifications Site Requirements 24 Physical Specifications Specifications 27 28 This chapter provides information on environmental requirements, physical and performance specifications.
2 Site Requirements and Specifications Site Requirements Site Requirements A suitable environment is important to ensure optimal performance of the module. Power Consideration The module power supply has wide ranging capabilities and accepts any line voltage in the range mentioned in Table 1 on page 27. Consequently, there is no voltage selector in the rear of the module. There are also no externally accessible fuses, because automatic electronic fuses are implemented in the power supply.
2 Site Requirements and Specifications Site Requirements Power Cords Different power cords are offered as options with the module. The female end of all power cords is identical. It plugs into the power- input socket at the rear. The male end of each power cord is different and designed to match the wall socket of a particular country or region.
2 Site Requirements and Specifications Site Requirements Bench Space The module dimensions and weight (see Table 1 on page 27) allow you to place the module on almost any desk or laboratory bench. It needs an additional 2.5 cm (1.0 inches) of space on either side and approximately 8 cm (3.1 inches) in the rear for air circulation and electric connections. If the bench shall carry a complete HPLC system, make sure that the bench is designed to bear the weight of all modules.
2 Site Requirements and Specifications Physical Specifications Physical Specifications Table 1 Physical Specifications Type Specification Weight 15.5 kg (35 lbs) Dimensions (height × width × depth) 200 x 345 x 440 mm (8 x 13.5 x 17 inches) Line voltage 100 – 240 VAC, ± 10 % Line frequency 50 or 60 Hz, ± 5 % Power consumption 200 VA / 200 W / 683 BTU Ambient operating temperature 4–55 °C (39–131 °F) Ambient non-operating temperature -40 – 70 °C (-40 – 158 °F) Humidity < 95 % r.h.
2 Site Requirements and Specifications Specifications Specifications Table 2 Performance Specifications (G1367E) Type Specification Injection range 0.1 – 100 µL in 0.1 µL increments. Up to 40 µL with reduced injection volume kit (hardware modification required). Up to 1500 µL with multiple draw (hardware modification required). Precision <0.25 % from 5 – 40 µL <0.5 % from 2 – 5 µL <0.7 % from 1 – 2 µL <1.5 % from 0.
2 Site Requirements and Specifications Specifications Table 2 Performance Specifications (G1367E) Type Specification Comment Local Control Agilent Instant Pilot (G4208A) B.02.11 or above Communications Controller-area network (CAN), RS-232C, APG Remote: ready, start, stop and shut-down signals, optional four external contact closures and BCD vial number out.
2 30 Site Requirements and Specifications Specifications Agilent 1260 Infinity High Performance Autosampler User Manual
Agilent 1260 Infinity High Performance Autosampler User Manual 3 Installing the Autosampler Unpacking the Autosampler 32 Damaged Packaging 32 Delivery Checklist 33 Autosampler Accessory Kit Contents Optimizing the Stack Configuration One Stack Configuration 36 Two Stack Configuration 38 34 35 Installation Information on Leak and Waste Handling Installing the Autosampler 40 44 Flow Connections to the Autosampler 46 This chapter provides information on unpacking, checking on completeness, stack consi
3 Installing the Autosampler Unpacking the Autosampler Unpacking the Autosampler Damaged Packaging If the delivery packaging shows signs of external damage, please call your Agilent Technologies sales and service office immediately. Inform your service representative that the instrument may have been damaged during shipment. CAUTION "Defective on arrival" problems If there are signs of damage, please do not attempt to install the module.
Installing the Autosampler Unpacking the Autosampler 3 Delivery Checklist Ensure all parts and materials have been delivered with the autosampler. For this compare the shipment content with the checklist included in each instrument box. Please report missing or damaged parts to your local Agilent Technologies sales and service office.
3 Installing the Autosampler Unpacking the Autosampler Autosampler Accessory Kit Contents 34 p/n Description G1367-68755 Accessory kit 5181-1519 CAN cable, Agilent module to module, 1 m G1367-87304 Capillary ST 0.17 mm x 250 mm S/S 01090-87306 Capillary ST 0.17 mm x 380 nm S/S G1329-43200 Adapter air channel 5063-6527 Tubing assembly, i.d. 6 mm, o.d. 9 mm, 1.
Installing the Autosampler Optimizing the Stack Configuration 3 Optimizing the Stack Configuration If your module is part of a complete Agilent 1260 Infinity Liquid Chromatograph, you can ensure optimum performance by installing the following configurations. These configurations optimize the system flow path, ensuring minimum delay volume.
3 Installing the Autosampler Optimizing the Stack Configuration One Stack Configuration Ensure optimum performance by installing the modules of the Agilent 1260 Infinity LC System in the following configuration (See Figure 7 on page 36 and Figure 8 on page 37). This configuration optimizes the flow path for minimum delay volume and minimizes the bench space required.
Installing the Autosampler Optimizing the Stack Configuration 3 GZbdiZ XVWaZ 86C 7jh XVWaZ id >chiVci E^adi 68 edlZg 86C 7jh XVWaZ 6cVad\ YZiZXidg h^\cVa & dg ' djiejih eZg YZiZXidg A6C id A8 8]ZbHiVi^dc adXVi^dc YZeZcYh dc YZiZXidg Figure 8 Recommended Stack Configuration for 1260 Infinity (Rear View) Agilent 1260 Infinity High Performance Autosampler User Manual 37
3 Installing the Autosampler Optimizing the Stack Configuration Two Stack Configuration To avoid excessive height of the stack when the autosampler thermostat is added to the system it is recommended to form two stacks. Some users prefer the lower height of this arrangement even without the autosampler thermostat. A slightly longer capillary is required between the pump and autosampler. (See Figure 9 on page 38 and Figure 10 on page 39).
Installing the Autosampler Optimizing the Stack Configuration 3 A6C id Xdcigda hd[ilVgZ 86C 7jh XVWaZ id >chiVci E^adi 6jidhVbeaZg$ ;gVXi^dc 8daaZXidg 8VWaZ GZbdiZ XVWaZ 86C 7jh XVWaZ 68 EdlZg Figure 10 Recommended Two Stack Configuration for 1260 Infinity (Rear View) Agilent 1260 Infinity High Performance Autosampler User Manual 39
3 Installing the Autosampler Installation Information on Leak and Waste Handling Installation Information on Leak and Waste Handling The Agilent 1200 Infinity Series has been designed for safe leak and waste handling. It is important that all security concepts are understood and instructions are carefully followed. WA R N I N G Toxic, flammable and hazardous solvents, samples and reagents The handling of solvents, samples and reagents can hold health and safety risks.
Installing the Autosampler Installation Information on Leak and Waste Handling 3 & 6 ' 7 8 ( ) * , + , Figure 11 Leak and waste handling (overview - typical stack configuration as an example) Agilent 1260 Infinity High Performance Autosampler User Manual 41
3 Installing the Autosampler Installation Information on Leak and Waste Handling 1 Solvent cabinet 2 Leak pan 3 Leak pan's outlet port (A), leak funnel (B) and corrugated waste tube (C) 4 Waste tube of the sampler’s needle wash 5 Condense drain outlet of the autosampler cooler 6 Waste tube of the purge valve 7 Waste tube 1 Stack the modules according to the adequate stack configuration.
Installing the Autosampler Installation Information on Leak and Waste Handling Figure 12 3 Warning label (illustration for correct waste tubing) Agilent 1260 Infinity High Performance Autosampler User Manual 43
3 Installing the Autosampler Installing the Autosampler Installing the Autosampler Parts required Description Autosampler Power cord Hardware required Other cables see below and section “Cable Overview” on page 156 Software required ChemStation and/or Instant Pilot G4208A with the appropriate revisions, see Table 2 on page 28 WA R N I N G Module is partially energized when switched off, as long as the power cord is plugged in. Repair work at the module can lead to personal injuries, e.g.
3 Installing the Autosampler Installing the Autosampler 3 Connect the power cable to the power connector at the rear of the module. Figure 13 Rearview of Autosampler 4 Connect the CAN cable to other Agilent 1260 Infinity modules. 5 Connect the APG remote cable (optional) for non- Agilent instruments. 6 Turn on the power by pushing the button at the lower left hand side of the module. The power button stays pressed in and the status LED should be green.
3 Installing the Autosampler Flow Connections to the Autosampler Flow Connections to the Autosampler Parts required Description System Capillaries and tubing from Accessory Kit. Preparations NOTE WA R N I N G Autosampler is installed in system.
Agilent 1260 Infinity High Performance Autosampler User Manual 4 Using the Module Leak and Waste Handling Preparing the Autosampler 48 49 Setting up the Autosampler with Agilent ChemStation Control Settings 55 Method Parameter Settings 56 Module Configuration 60 51 Main Screens of the Autosampler with Agilent Instant Pilot (G4208A) 61 This chapter provides information on how to set up the autosampler for an analysis and explains the basic settings.
4 Using the Module Leak and Waste Handling Leak and Waste Handling WA R N I N G Toxic, flammable and hazardous solvents, samples and reagents The handling of solvents, samples and reagents can hold health and safety risks. ➔ When working with these substances observe appropriate safety procedures (for example by wearing goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet supplied by the vendor, and follow good laboratory practice.
Using the Module Preparing the Autosampler 4 Preparing the Autosampler For best performance of the autosampler • When using the Autosampler in a system with a vacuum degassing unit, shortly degas your samples before using them in the autosampler. • Filter samples before use in 1260 system. Use High pressure filter kit (5067- 4638) for inline filtering. • When using buffer solutions, flush the system with water before switching it off.
4 Using the Module Preparing the Autosampler • Priming and Purging the System - When the solvents have been exchanged or the system has been turned off for a certain time (for example, overnight) oxygen will re- diffuse into the solvent channel. Therefore priming and purging of the system is required before starting an application.
Using the Module Setting up the Autosampler with Agilent ChemStation 4 Setting up the Autosampler with Agilent ChemStation The setup of the Autosampler is shown with the Agilent ChemStation B.04.02. SP1 DSP3. Depending on the controller (e.g. Agilent Instant Pilot, EZChrom Elite) the screens look different. For the Instant Pilot refer to “Main Screens of the Autosampler with Agilent Instant Pilot (G4208A)” on page 61. NOTE This section describes the autosampler settings only.
4 Using the Module Setting up the Autosampler with Agilent ChemStation Figure 14 ChemStation Method & Run Control After successful load of the ChemStation, you should see the module as an active item in the graphical user interface (GUI).
Using the Module Setting up the Autosampler with Agilent ChemStation 4 The Autosampler User Interface & ( ) Within the Autosampler user interface, there are active areas.
4 Using the Module Setting up the Autosampler with Agilent ChemStation Module Status shows Run / Ready / Error state and “Not Ready text” or “Error text”. • Error (Red) • Not ready (yellow) • Ready (green) • Pre run, Post run (purple) • Run (blue) • Idle (green) • Offline (dark gray) • Standby (light gray) EMF Status shows Run / Ready / Error state and “Not Ready text” or “Error text”. • Offline (gray) • Ok. No Maintenance required (green) • EMF warning.
Using the Module Setting up the Autosampler with Agilent ChemStation 4 Control Settings These settings are available via right click on the Active Area of the ALS GUI. Missing Vessel: The handling of missing vessels can be configured. Linked Pump: To configure which pump delivers flow to the Autosampler. Prime Flush Pump: Priming the Needle wash flush pump.
4 Using the Module Setting up the Autosampler with Agilent ChemStation Method Parameter Settings These settings are available via Menu > Instrument > Setup Agilent 1260 Infinity Autosampler or via right click on the Active area. NOTE The signal window in the lower part is not shown when opening the parameter settings via right mouse on the Autosampler user interface.
Using the Module Setting up the Autosampler with Agilent ChemStation 4 Injection Mode The settable Injection volume range is from 0.1 – 20.0 µL. Select to use Standard injection or Injection with Needle wash. Needle wash It is possible to select between using the built in flush port of the Autosampler or using a non-capped vial. Using needle wash is required to obtain minimum carry-over. Stop Time An autosampler Stop Time can be set.
4 Using the Module Setting up the Autosampler with Agilent ChemStation Injection Cleaning The Injection Valve Cleaning section allows you to specify the valve switching times at the end of overlap or sample flush. Times 1 ... 4 are the times when the valve switches to bypass (for time 1) or to mainpass and bypass (for times 2, 3 and 4).The times must be specified in ascending order. You can also switch the times to off.
Using the Module Setting up the Autosampler with Agilent ChemStation 4 Injection Program The pretreatment/injector program comprises a series of numbered lines, each specifying an operation that the autosampler carries out sequentially. When you activate a pretreatment/injector program, it replaces the standard injection cycle. Select Append to add the contents of the edit line to the end of the table. Select Insert to insert the contents of the edit line above the currently-selected line.
4 Using the Module Setting up the Autosampler with Agilent ChemStation Module Configuration These settings are available via menu Instrument > More 1260 Infinity ALS > Autosampler Configuration. Device name: based on the module. Type ID: based on the module (product number). Some modules may allow changing the type based on hardware/firmware. This results in a change of features and functions. Serial number: based on the module. Firmware revision: based on the module. Options: lists installed options.
Using the Module Main Screens of the Autosampler with Agilent Instant Pilot (G4208A) 4 Main Screens of the Autosampler with Agilent Instant Pilot (G4208A) Below the main screens for the use of the autosampler are shown.
4 Using the Module Main Screens of the Autosampler with Agilent Instant Pilot (G4208A) The Configure screen allows to configure • Symbolic Name of module • Volumes • On Missing Vessel behaviour • Plate configuration • Flush-Out Pump • Serial Interface configuration • Sample Illumination The Method screen lists all method parameters of the autosampler. These can be edited.
Using the Module Main Screens of the Autosampler with Agilent Instant Pilot (G4208A) 4 The Maintenance screen allows • EMF setup • logging of maintenance activities • module identification (blinking LED) Firmware updates can be done via the System Maintenance screen. The Diagnosis screen provides access to module specific tests.
4 64 Using the Module Main Screens of the Autosampler with Agilent Instant Pilot (G4208A) Agilent 1260 Infinity High Performance Autosampler User Manual
Agilent 1260 Infinity High Performance Autosampler User Manual 5 Optimizing Performance Delay Volume and Extra-Column Volume Delay Volume 66 66 How to Configure the Optimum Delay Volume How to Achieve Higher Injection Volumes How to Achieve High Throughput 67 70 72 How to Achieve Higher Resolution 73 How to Achieve Higher Sensitivity 76 How to Achieve Lowest Carry Over 77 This chapter gives hints on how to optimize the performance or use additional devices.
5 Optimizing Performance Delay Volume and Extra-Column Volume Delay Volume and Extra-Column Volume The delay volume is defined as the system volume between the point of mixing in the pump and the top of the column. The extra- column volume is defined as the volume between the injection point and the detection point, excluding the volume in the column. Delay Volume In gradient separations, this volume causes a delay between the mixture changing in the pump and that change reaching the column.
5 Optimizing Performance How to Configure the Optimum Delay Volume How to Configure the Optimum Delay Volume For very fast gradients over 0.5 min the delay volume of the system can be easily reduced without changing the physical configuration of the system. The change is achieved by changing the behavior of the autosampler.
5 Optimizing Performance How to Configure the Optimum Delay Volume &# KVakZ ^c bV^ceVhh! [adl i]gdj\] '# KVakZ ^c WneVhh! YgVl^c\ hVbeaZ (# KVakZ ^c WneVhh! lVh]^c\ cZZYaZ )# KVakZ ^c bV^ceVhh! hVbeaZ ^c_ZXiZY Figure 16 Schematic of injection steps in 1260 Infinity Autosampler When using ADVR it should be noted that the gradient has already started at the pump at the instant of injection.
Optimizing Performance How to Configure the Optimum Delay Volume 5 The ADVR function is unlikely to be suitable for applications involving compounds which are known to cause carry- over problems. The best solution to reduce the delay volume is to install the 40 µL injection upgrade kit (G4215A). The standard metering device is replaced by a 40 µL Micro Analytical Head and a new 40 µL Loop must be installed.
5 Optimizing Performance How to Achieve Higher Injection Volumes How to Achieve Higher Injection Volumes The standard configuration of the Agilent 1260 Infinity Autosampler can inject a maximum volume of 100 µL with the standard loop capillary. To increase the injection volume the Multidraw upgrade kit (G1313- 68711) can be installed. With the kit you can add a maximum of 400 µL or 1400 µL to the injection volume of your injector.
Optimizing Performance How to Achieve Higher Injection Volumes 5 but it is very dependent on the chemistry of the analyte and mobile phase as discussed above. In a gradient separation injection volumes of about 5 % of the column volume might be achieved whilst maintaining good resolution and peak dispersion. One way to achieve larger injections is to use a trapping column selected by a switching valve to capture and concentrate the injection before switching it, i.e.
5 Optimizing Performance How to Achieve High Throughput How to Achieve High Throughput The injection can be optimized for speed remembering that drawing the sample too fast can reduce the reproducibility. Marginal gains are to be made here as the sample volumes used tend towards the smaller end of the range in any case. A significant portion of the injection time is the time taken with the needle movements to and from the vial and into the flush port.
5 Optimizing Performance How to Achieve Higher Resolution How to Achieve Higher Resolution Increased resolution in a separation will improve the qualitative and quantitative data analysis, allow more peaks to be separated or offer further scope for speeding up the separation.
5 Optimizing Performance How to Achieve Higher Resolution The resolution equation shows that the next most significant term is the plate count or efficiency, N, and this can be optimized in a number of ways. N is inversely proportional to the particle size and directly proportional to the length of a column and so smaller particle size and a longer column will give a higher plate number. The pressure rises with the inverse square of the particle size and proportionally with the length of the column.
Optimizing Performance How to Achieve Higher Resolution 5 In isocratic separations, increasing the retention factor, k, results in better resolution because the solute is retained longer. In gradient separations the retention is described by k* in the following equation: where: • k* = mean k value, • tG = time length of gradient (or segment of gradient) (min), • F = flow (ml/min), • Vm = column delay volume, • Δ%B = change in fraction of solvent B during the gradient, • S = constant (ca.
5 Optimizing Performance How to Achieve Higher Sensitivity How to Achieve Higher Sensitivity The sensitivity of a separation method is linked to the choice of stationary and mobile phases as good separation with narrow peaks and a stable baseline with minimal noise are desirable. The choice of instrument configuration will have an effect and a major impact is the setup of the detector.
Optimizing Performance How to Achieve Lowest Carry Over 5 How to Achieve Lowest Carry Over Carryover is measured when residual peaks from a previous active- containing injection appear in a subsequent blank solvent injection. There will be carry over between active injections which may lead to erroneous results. The level of carryover is reported as the area of the peak in the blank solution expressed as a percentage of the area in the previous active injection.
5 Optimizing Performance How to Achieve Lowest Carry Over The flush port is located above and behind the needle seat and a peristaltic pump delivers the wash solvent. It has a volume of 0.68 ml and the peristaltic pump delivers 6 ml/min, which means the flush port volume is completely refilled with fresh solvent in 7 s. If the flush port is selected, the user can set how long the outside of the needle is to be washed with fresh solvent.
Agilent 1260 Infinity High Performance Autosampler User Manual 6 Troubleshooting and Diagnostics Overview of the Module’s Indicators and Test Functions 80 Status Indicators 81 Power Supply Indicator 81 Module Status Indicator 82 User Interfaces 83 Agilent Lab Advisor Software 84 This chapter gives an overview about the troubleshooting and diagnostic features and the different user interfaces.
6 Troubleshooting and Diagnostics Overview of the Module’s Indicators and Test Functions Overview of the Module’s Indicators and Test Functions Status Indicators The module is provided with two status indicators which indicate the operational state (prerun, run, and error states) of the module. The status indicators provide a quick visual check of the operation of the module.
Troubleshooting and Diagnostics Status Indicators 6 Status Indicators Two status indicators are located on the front of the module. The lower left indicates the power supply status, the upper right indicates the module status. Figure 18 Location of Status Indicators Power Supply Indicator The power supply indicator is integrated into the main power switch. When the indicator is illuminated (green) the power is ON.
6 Troubleshooting and Diagnostics Status Indicators Module Status Indicator The module status indicator indicates one of six possible module conditions: • When the status indicator is OFF (and power switch light is on), the module is in a prerun condition, and is ready to begin an analysis. • A green status indicator, indicates the module is performing an analysis (run mode). • A yellow indicator indicates a not- ready condition.
Troubleshooting and Diagnostics User Interfaces 6 User Interfaces • Depending on the user interface, the available tests and the screens/reports may vary. • Preferred tool should be Agilent Lab Advisor Software, see “Agilent Lab Advisor Software” on page 84. • The Agilent ChemStation B.04.02 and above do not include any maintenance/test functions. • Screenshots used within these procedures are based on the Agilent Lab Advisor Software.
6 Troubleshooting and Diagnostics Agilent Lab Advisor Software Agilent Lab Advisor Software The Agilent Lab Advisor software is a standalone product that can be used with or without data system. Agilent Lab Advisor software helps to manage the lab for high quality chromatographic results and can monitor in real time a single Agilent LC or all the Agilent GCs and LCs configured on the lab intranet. Agilent Lab Advisor software provides diagnostic capabilities for all Agilent 1200 Infinity Series modules.
Agilent 1260 Infinity High Performance Autosampler User Manual 7 Error Information What are Error Messages 87 General Error Messages 88 Timeout 88 Shutdown 88 Remote Timeout 89 Lost CAN Partner 89 Leak 90 Leak Sensor Open 90 Leak Sensor Short 91 Compensation Sensor Open Compensation Sensor Short Fan Failed 92 91 92 Module Error Messages 93 Exhaust Fan Failed 93 Front Door Error 93 Side Door Error 94 Arm Movement Failed or Arm Movement Timeout Valve to Bypass Failed 95 Valve to Mainpass Failed 95 Needle
7 Error Information Agilent Lab Advisor Software Vessel or Wash Vessel Error 103 Vessel Stuck to Needle 104 Rear Blind Seat Missing 104 This chapter describes the meaning of error messages, and provides information on probable causes and suggested actions how to recover from error conditions.
Error Information What are Error Messages 7 What are Error Messages Error messages are displayed in the user interface when an electronic, mechanical, or hydraulic (flow path) failure occurs which requires attention before the analysis can be continued (for example, repair, or exchange of consumables is necessary). In the event of such a failure, the red status indicator at the front of the module is switched on, and an entry is written into the module logbook.
7 Error Information General Error Messages General Error Messages General error messages are generic to all Agilent series HPLC modules and may show up on other modules as well. Timeout Error ID: 0062 The timeout threshold was exceeded. Probable cause Suggested actions 1 The analysis was completed successfully, Check the logbook for the occurrence and source of a not-ready condition. Restart the analysis where required. and the timeout function switched off the module as requested.
Error Information General Error Messages 7 Remote Timeout Error ID: 0070 A not- ready condition is still present on the remote input. When an analysis is started, the system expects all not- ready conditions (for example, a not- ready condition during detector balance) to switch to run conditions within one minute of starting the analysis. If a not- ready condition is still present on the remote line after one minute the error message is generated.
7 Error Information General Error Messages Leak Error ID: 0064 A leak was detected in the module. The signals from the two temperature sensors (leak sensor and board- mounted temperature- compensation sensor) are used by the leak algorithm to determine whether a leak is present. When a leak occurs, the leak sensor is cooled by the solvent. This changes the resistance of the leak sensor which is sensed by the leak- sensor circuit on the main board. Probable cause Suggested actions 1 Loose fittings.
Error Information General Error Messages 7 Leak Sensor Short Error ID: 0082 The leak sensor in the module has failed (short circuit). The current through the leak sensor is dependent on temperature. A leak is detected when solvent cools the leak sensor, causing the leak sensor current to change within defined limits. If the current increases above the upper limit, the error message is generated. Probable cause Suggested actions 1 Defective leak sensor.
7 Error Information General Error Messages Compensation Sensor Short Error ID: 0080 The ambient- compensation sensor (NTC) on the main board in the module has failed (open circuit). The resistance across the temperature compensation sensor (NTC) on the main board is dependent on ambient temperature. The change in resistance is used by the leak circuit to compensate for ambient temperature changes. If the resistance across the sensor falls below the lower limit, the error message is generated.
Error Information Module Error Messages 7 Module Error Messages These errors are autosampler specific. Exhaust Fan Failed Error ID: 4456, 4457 The exhaust fan in the module has failed. The hall sensor on the fan shaft is used by the main board to monitor the fan speed. If the fan speed falls below a certain value the error message is generated and the module shuts down. Probable cause Suggested actions 1 Fan cable disconnected. Please contact your Agilent service representative. 2 Defective fan.
7 Error Information Module Error Messages Side Door Error Error ID: 4355, 4459 The side door and/or the main board are damaged. Probable cause Suggested actions 1 The door is bent or the magnet is Please contact your Agilent service representative. misplaced/broken. 2 The sensor on the main board is defective. Please contact your Agilent service representative. Arm Movement Failed or Arm Movement Timeout Error ID: 4002 The transport assembly was unable to complete a movement in one of the axes.
Error Information Module Error Messages 7 Valve to Bypass Failed Error ID: 4014, 4701 The injection valve failed to switch to the bypass position. The switching of the injection valve is monitored by two microswitches on the valve assembly. The switches detect the successful completion of the valve movement. If the valve fails to reach the bypass position, or if the microswitch does not close, the error message is generated.
7 Error Information Module Error Messages Needle Lock Failed Error ID: 4702, 4703 The lock assembly on the sampling unit failed to move successfully. The upper and lower positions of the needle lock are monitored by position sensors on the sampling unit flex board. The sensors detect the successful completion of the needle lock movement. If the needle lock fails to reach the end point, or if the sensors fail to recognize the needle lock movement, the error message is generated.
Error Information Module Error Messages 7 Needle to Needle Seat Position Error ID: 4510, 4511, 4714 The needle failed to reach the end position in the needle seat. The position of the needle is monitored by a position encoder on the needle carrier. If the needle fails to reach the end point, or if the encoder fails to recognize the needle carrier movement, the error message is generated.
7 Error Information Module Error Messages Missing Vial or Missing Wash Vial Error ID: 4019, 4034, 4035, 4541, 4542, 4706, 4707 No vial was found in the position defined in the method or sequence. When the needle carrier moves to a vial and the needle goes into the vial, the position of the needle is monitored by an encoder behind the vial pusher. If no vial is present, the encoder detects an error and the message “missing vial” is generated.
Error Information Module Error Messages 7 Initialization Failed Error ID: 4020 The autosampler failed to complete initialization correctly. The autosampler initialization procedure moves the needle arm and transport assembly to their home positions in a predefined routine. During initialization, the processor monitors the position sensors and motor encoders to check for correct movement. If one or more of the movements is not successful, or is not detected, the error message is generated.
7 Error Information Module Error Messages Metering Home Failed Error ID: 4054, 4704 The metering piston has failed to move back to the home position. The home position sensor on the sampling unit flex board monitors the home position of the piston. If the piston fails to move to the home position, or if the sensor fails to recognize the piston position, the error message is generated. 100 Probable cause Suggested actions 1 Dirty or defective sensor. Please contact your Agilent service representative.
Error Information Module Error Messages 7 Motor Temperature Error ID: 4027, 4040, 4261, 4451 One of the motors of the transport assembly has drawn excessive current, causing the motor to become too hot. The processor has switched off the motor to prevent damage to the motor. Motor identification: • Motor 0 temperature: X- axis motor. • Motor 1 temperature: Z- axis motor. • Motor 2 temperature: Theta motor. The processor monitors the current drawn by each motor and the time the motor is drawing current.
7 Error Information Module Error Messages Invalid Vial Position Error ID: 4042 The vial position defined in the method or sequence does not exist. The reflection sensors on the transport assembly flex board are used to automatically check which sample trays are installed (coding on tray). If the vial position does not exist in the current sample tray configuration, the error message is generated. Probable cause Suggested actions 1 Incorrect tray installed.
Error Information Module Error Messages 7 Peristaltic Pump Error Error ID: 4514 The peristaltic pump motor in the autosampler has failed. The current on the motor is used by the MTP board to monitor the speed of the peristaltic pump motor. If the current falls below a certain value, the error message is generated. Probable cause Suggested actions 1 Defective motor. Please contact your Agilent service representative. 2 Defective SUD board. Please contact your Agilent service representative.
7 Error Information Module Error Messages Vessel Stuck to Needle Error ID: 4453 The vessel sticks to the needle when the needle moves up. Probable cause Suggested actions 1 Closing mat to rigid/thick. Check that the closing mat is not too thick. 2 Bad X or Theta positioning and the needle Please contact your Agilent service representative. sticks into the wall between two holes. 3 Defective encoder on the needle carrier assembly. Please contact your Agilent service representative.
Agilent 1260 Infinity High Performance Autosampler User Manual 8 Test Functions Introduction 106 System Pressure Test 107 System Pressure Test Evaluation Sample Transport Self Alignment 109 110 Maintenance Positions 112 Change Needle 113 Change Loop Capillary 113 Arm Position 114 Change Needle Carrier 114 Change Metering Device 115 Injector Steps 116 Step Commands 117 This chapter describes the tests for the module.
8 Test Functions Introduction Introduction All tests are described based on the Agilent Lab Advisor Software B.01.04 or above. Other user interfaces may not provide any test or just a few.
Test Functions System Pressure Test 8 System Pressure Test The test determines the leak rate of the system between pump outlet valves and a blank nut. The blank nut can be positioned at different locations in the system before the flow cell, to determine and verify the leak rate of individual modules and components. The test allows for setting the pressure at which the test is performed.
8 Test Functions System Pressure Test 1 Run the System pressure test with the Agilent Lab Advisor (for further information see Online- Help of user interface).
Test Functions System Pressure Test 8 System Pressure Test Evaluation System Pressure Test Failed Probable cause Suggested actions 1 Pump leakages Perform the Pump Head Leak test. 2 Loose or leaky fittings Tighten the fittings or replace capillaries. 3 Autosampler leakages Perform the Autosampler Leak test. 4 Thermostatted Column Compartment valve Replace the TCC valve rotor seal.
8 Test Functions Sample Transport Self Alignment Sample Transport Self Alignment The sample transport self alignment uses predefined positions on the well plate tray to calibrate the positioning of the needle. The sample transport self alignment is required to compensate for larger deviations in positioning the needle carrier. The sample transport self alignment is required after disassembling the system or when you exchange the sample transport, the sampling unit, the tray or the MTP main board.
Test Functions Sample Transport Self Alignment 8 1 Run the Transport Alignment with the Agilent Lab Advisor (for further information see Online- Help of user interface).
8 Test Functions Maintenance Positions Maintenance Positions Some maintenance procedures require the needle arm, metering device, and needle carrier to be moved to specific positions to enable easy access to components. The maintenance functions move these assemblies into the appropriate maintenance position. In the Agilent Lab Advisor Software the maintenance positions can be selected from the Tools icon. When When performing Maintenance on the module.
Test Functions Maintenance Positions 8 Change Needle The position is positioning the needle carrier so that there is easy access for changing needle or needle seat. The position is to the far left, and the current to the motors are off, so that the arm can be turned while servicing the module. Figure 23 Maintenance Positions– Change Needle Change Loop Capillary The Change Loop Capillary command positions the arm in the middle of the tray at half height to enable easy exchange of the loop cartridge.
8 Test Functions Maintenance Positions Arm Position The home position of the autosampler ensures a better access to the tray area and for exchanging trays. When transporting the module it is highly recommended to use the Park Arm command, in order to place the Arm in a position for safe transport. Figure 25 Maintenance Positions– Arm Position Change Needle Carrier The Change Needle Carrier function moves the needle to the front of the autosampler, enabling easy access to the needle carrier mechanism.
8 Test Functions Maintenance Positions Change Metering Device When removing the metering device is necessary (by exchanging the metering seal for instance), the metering drive needs to be moved to a position at the far back, in order to prevent seal and/or piston damage.
8 Test Functions Injector Steps Injector Steps Each movement of the sampling sequence can be done under manual control. This is useful during troubleshooting, where close observation of each of the sampling steps is required to confirm a specific failure mode or verify successful completion of a repair. Each injector step command actually consists of a series of individual commands that move the autosampler components to predefined positions, enabling the specific step to be done.
8 Test Functions Injector Steps Step Commands Table 5 Step Commands Step Action Valve Bypass Switches injection valve to the bypass position. Plunger Home Moves the plunger to the home position. Needle Up Lifts the needle arm to the upper position. Move to Location Move the needle arm to the vial location on the plate. Needle into Sample Lowers the needle into the vial. Draw Metering device draws the defined injection volume. Needle Up Lifts the needle out of the vial.
8 118 Test Functions Injector Steps Agilent 1260 Infinity High Performance Autosampler User Manual
Agilent 1260 Infinity High Performance Autosampler User Manual 9 Maintenance Introduction to Maintenance Warnings and Cautions 121 Overview of Maintenance Cleaning the module 120 122 123 Removing the needle assembly 124 Installing the needle assembly 127 Exchanging the Needle Seat Replacing the Rotor seal 130 132 Removing the Metering Seal 135 Installing the Metering Seal 138 Replacing Peristaltic Pump Cartridge Installing the Interface Board Replacing the Module Firmware 140 143 144 Th
9 Maintenance Introduction to Maintenance Introduction to Maintenance Figure 29 on page 120 shows the main user accessible assemblies of the autosampler. These parts can be accessed from the front (simple repairs) and don't require to remove the autosampler from the system stack.
9 Maintenance Warnings and Cautions Warnings and Cautions WA R N I N G Toxic, flammable and hazardous solvents, samples and reagents The handling of solvents, samples and reagents can hold health and safety risks. ➔ When working with these substances observe appropriate safety procedures (for example by wearing goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet supplied by the vendor, and follow good laboratory practice.
9 Maintenance Overview of Maintenance Overview of Maintenance The following pages describe maintenance (simple repairs) of the autosampler that can be carried out without opening the main cover. Table 6 Overview of Maintenance Procedure Typical Frequency Change needle/needle seat 60.000 needle into seat Change metering seal 30.000 injections Peristaltic pump cartridge 3000 hours on-time Change rotor seal 30.
9 Maintenance Cleaning the module Cleaning the module To keep the module case clean, use a soft cloth slightly dampened with water, or a solution of water and mild detergent. WA R N I N G Liquid dripping into the electronic compartment of your module can cause shock hazard and damage the module ➔ Do not use an excessively damp cloth during cleaning. ➔ Drain all solvent lines before opening any connections in the flow path.
9 Maintenance Removing the needle assembly Removing the needle assembly When When the limit in the needle into seat counter in the EMF is exceeded or when needle shows indications of damage, blockage or leaks. Tools required p/n Description 8710-0510 Wrench open 1/4 — 5/16 inch Parts required Preparations WA R N I N G p/n Description G4226-87201 Needle assembly In order to avoid leaks, close the shutoff valves in the pump or remove tubings from solvent bottles.
Maintenance Removing the needle assembly 1 In the user interface start the maintenance mode and 9 2 Open the front door and remove the side door. select Change needle/seat function. In the Agilent Lab Advisor software the Change needle/seat function can be found in the Tools section. 3 Turn the Needle carrier 90 ° clockwise. 4 Flip the Leak guide open.
9 Maintenance Removing the needle assembly 5 Attach a 5/16 inch wrench to hold the position at the needle assembly. Use a 1/4 inch wrench to loosen the fitting of the loop capillary. 6 Pinch the holder clamp, pull back and remove the loop capillary from the needle assembly. 7 Remove the needle assembly.
Maintenance Installing the needle assembly 9 Installing the needle assembly When When the limit in the needle into seat counter in the EMF is exceeded or when needle shows indications of damage, blockage or leaks. Tools required p/n Description 8710-0510 Wrench open 1/4 — 5/16 inch Parts required Preparations WA R N I N G p/n Description G4226-87201 Needle assembly In order to avoid leaks, close the shutoff valves in the pump or remove tubings from solvent bottles.
9 Maintenance Installing the needle assembly 1 Push the silicon safety tube delivered with every needle over the needle. tighten the fitting hand tight. 3 Pinch the holder clamp and reinsert the needle assembly into the needle carrier. 128 2 Insert the loop capillary into the needle assembly and 4 Attach a 5/16 inch wrench to hold the position at the needle assembly. Use a 1/4 inch wrench to tighten the fitting of the loop capillary.
9 Maintenance Installing the needle assembly 5 Close the leak guide Next Steps: 6 Check the alignment of the needle in the needle pusher of the needle carrier by viewing from several directions to see that it is aligned in the center of the needle pusher. NOTE The needle must be centered in the needle pusher as all alignment by the Autosampler is calculated from the needle pusher position. 7 Remove the silicon safety tube from the needle.
9 Maintenance Exchanging the Needle Seat Exchanging the Needle Seat When When seat is visibly damaged, blocked or leaks. Tools required p/n Description 8710-0510 ¼ inch wrench Flat head screwdriver Parts required Preparations WA R N I N G # p/n Description 1 G1367-87012 Needle seat In order to avoid leaks, close the shutoff valves in the pump or remove tubings from solvent bottles. Risk of injury by uncovered needle An uncovered needle is a risk of harm to the operator.
Maintenance Exchanging the Needle Seat 3 Disconnect the seat capillary from the Injection valve. 9 4 With a Flat head screw driver carefully lift out the needle seat from the holder. Next Steps: 5 Insert the new needle seat. Press it firmly in position. 6 In the user interface exit the Change needle/seat function and exit the maintenance mode. In the Lab Advisor software the Change needle/seat function can be found in the Tools section.
9 Maintenance Replacing the Rotor seal Replacing the Rotor seal When When poor injection volume reproducibility or when injection valve is leaking. Tools required p/n Description 8710-0510 ¼ inch wrench 8710-2394 9/64 inch hex key Parts required 1 Open the front door. # p/n Description 1 0101-1416 Injection valve rotor seal 2 Remove all capillaries from the Injection valve with a 1/4 inch wrench.
Maintenance Replacing the Rotor seal 3 Unscrew and remove the three stator screws from the 9 4 Remove the stator head and the stator ring. stator head with a 9/64 inch hex key. 5 Remove the rotor seal. 6 Remove the Isolation seal.
9 Maintenance Replacing the Rotor seal 7 Install new rotor seal and isolation seal. 8 Reinstall the stator ring and stator head. The pins on stator ring and stator head must engage in the corresponding holes. 9 Insert and tighten the stator screws alternating with the Next Steps: 9/64 inch hex key, until the stator head is secure. 10 Reconnect all capillaries to the injection valve ports with a 1/4 inch wrench. The positions of the individual fittings can be seen on the sticker on the sampling unit.
9 Maintenance Removing the Metering Seal Removing the Metering Seal When When poor injection volume reproducibility or when metering device / analytical head is leaking. Tools required p/n Description 8710-0510 Wrench open 1/4 — 5/16 inch 8710-2392 4 mm Hex key G4226-43800 Seal insert tool Parts required # p/n Description 1 5063-6589 Metering seal (pack of 2) for 100 µL analytical head 1 In the user interface start the maintenance mode and 2 Open the front door.
9 Maintenance Removing the Metering Seal 3 Remove the two attached capillaries with a ¼ inch wrench. 4 Unscrew alternately the two fixing screws with a 4 mm hex key. 5 Pull the metering device / analytical head away from the 6 Remove the two fixing screws at the base of the metering sampling unit. 136 device / analytical head.
Maintenance Removing the Metering Seal 7 Remove the head body. 9 8 Carefully remove the metering seal using the steel side of the insert tool. Clean the chamber and ensure all particulate matter is removed.
9 Maintenance Installing the Metering Seal Installing the Metering Seal When When poor injection volume reproducibility or when metering device / analytical head is leaking. Tools required p/n Description 8710-0510 Wrench open 1/4 — 5/16 inch 8710-2392 4 mm Hex key G4226-43800 Seal insert tool Parts required Preparations # p/n Description 1 5063-6589 Metering seal (pack of 2) for 100 µL analytical head Removing the metering seal, see “Removing the Metering Seal” on page 135.
9 Maintenance Installing the Metering Seal 3 Press the piston into the seal. 4 Reinstall the metering device / analytical head to the sampling unit by tightening the two fixing screws alternately with a 4 mm hex key. 5 Connect the two capillaries to the metering device using Next Steps: a 1/4 inch wrench. 6 Close the front door. 7 In the user interface exit the Change Metering device function and exit the maintenance mode.
9 Maintenance Replacing Peristaltic Pump Cartridge Replacing Peristaltic Pump Cartridge When Tubing blocked or broken Parts required NOTE # p/n Description 1 5065-4445 Peristaltic pump cartridge The peristaltic pump cartridge is a replaceable unit. The tubing inside the pump is not replaceable. 1 Remove the corrugated leak tubing. 2 Press the two clips on the front of the peristaltic pump cartridge.
9 Maintenance Replacing Peristaltic Pump Cartridge 3 Pull the cartridge forward off the motor shaft. 4 Disconnect the tubing leading to the wash port and the tubing coming from the solvent bottle. 5 Connect the wash port tubing to the upper tubing of the new cartridge (use sand paper to get a good grip on the tubing). 6 Connect the tubing coming from the solvent bottle to the lower tubing of the new cartridge.
9 Maintenance Replacing Peristaltic Pump Cartridge 7 Push the cartridge onto the motor shaft until the clips 8 Reinstall the corrugated leak tubing. click into place.
Maintenance Installing the Interface Board 9 Installing the Interface Board When Tools required At installation or when defective. Description Flat head screwdriver Parts required CAUTION # Description 1 Interface board Electronic boards are sensitive to electrostatic discharge (ESD) and should be handled with care so as not to damage them. Touching electronic boards and components can cause electrostatic discharge. ESD can damage electronic boards and components.
9 Maintenance Replacing the Module Firmware Replacing the Module Firmware When The installation of newer firmware might be necessary • if a newer version solves problems of older versions or • to keep all systems on the same (validated) revision. The installation of older firmware might be necessary • to keep all systems on the same (validated) revision or • if a new module with newer firmware is added to a system or • if third party control software requires a special version.
Agilent 1260 Infinity High Performance Autosampler User Manual 10 Parts for Maintenance Overview of Maintenance Parts Vial Trays 146 147 Recommended Plates and Closing Mats Recommended Vial Plates Kits 148 149 150 Analytical Head Assembly Injection Valve Assembly Cover Parts 151 152 153 Leak System Parts 154 This chapter provides information on parts material required for the module.
10 Parts for Maintenance Overview of Maintenance Parts Overview of Maintenance Parts 146 Item p/n Description 1 0101-1416 Injection valve rotor seal 2 5063-6589 Metering seal (pack of 2) for 100 µL analytical head 3 G4226-87201 Needle assembly 4 G1367-87012 Needle seat 5 5067-4710 100 µL Flex Loop Kit 6 G1367-60003 Analytical head assembly (100 µL) Agilent 1260 Infinity High Performance Autosampler User Manual
Parts for Maintenance Vial Trays 10 Vial Trays + * & ) ( ' , Item p/n Description 1 G2258-60011 Tray for 2 plates + 10 x 2 mL vials 2 0515-0866 Screws for springs 3 G1313-09101 Spring 4 0570-1574 Spring stud 5 G4226-60000 Tray Support 6 G1329-43200 Adapter air channel G1367-47200 Plug channel G4226-60021 Tray for 100 micro vials 7 Agilent 1260 Infinity High Performance Autosampler User Manual 147
10 Parts for Maintenance Recommended Plates and Closing Mats Recommended Plates and Closing Mats Table 7 Recommended plates and closing mat Description (Part Number) Rows Columns Plate height Volume (μL) Package 384Agilent (5042-1388) 16 24 14.4 80 30 384Corning (No Agilent PN) 16 24 14.4 80 384Nunc (No Agilent PN) 16 24 14.4 80 96 well plate 0.5 ml, PP (pack of 10) (5042-1386) 96 well plate 0.5 ml, PP (pack of 120) (5042-1385) 8 12 14.
Parts for Maintenance Recommended Vial Plates 10 Recommended Vial Plates p/n Description G2255-68700 Vial plate for 54 x 2 mL vials (6/pk) 5022-6539 Vial plate for 15 x 6 mL vials (1/pk) 5022-6538 Vial plate for 27 Eppendorf tubes (1/pk) Agilent 1260 Infinity High Performance Autosampler User Manual 149
10 Parts for Maintenance Kits Kits Accessory Kit p/n Description G1367-68755 Accessory kit 5181-1519 CAN cable, Agilent module to module, 1 m G1367-87304 Capillary ST 0.17 mm x 250 mm S/S 01090-87306 SS Capillary 380 mmx 0.17 mm G1329-43200 Adapter air channel 5063-6527 Tubing assembly, i.d. 6 mm, o.d. 9 mm, 1.2 m (to waste) Injection Ugrade Kit Upgrade Kit for higher precision. 1260 HiP Autosampler option for RRLC configuration. The kit includes 40 µL analytical head and flex- loop kit.
Parts for Maintenance Analytical Head Assembly 10 Analytical Head Assembly & ' ( ) * Figure 30 Item Analytical Head Assembly p/n Description G1367-60003 Analytical head assembly (100 µL) 1 0515-0850 Screws 2 5063-6586 Piston 3 5001-3739 Support Seal assembly 4 5063-6589 Metering seal (pack of 2) for 100 µL analytical head 5 01078-27710 Head body 6 G4226-60301 Metering capillary SST Cap. 0.17 mm i.d.
10 Parts for Maintenance Injection Valve Assembly Injection Valve Assembly & ' ( ) * + 152 Item p/n Description 1 0101-1422 Injection valve 2 0100-1852 Isolation seal 3 5068-0118 Stator ring 4 0101-1416 Rotor seal (PEEK) 5 0101-1417 Stator head 6 5068-0018 Stator screws Agilent 1260 Infinity High Performance Autosampler User Manual
Parts for Maintenance Cover Parts 10 Cover Parts & Item p/n Description 1 5067-4662 Cabinet kit (base, sides and top) 5043-0207 Name plate 1260 G4226-67001 Door repair kit, includes the front door Agilent 1260 Infinity High Performance Autosampler User Manual 153
10 Parts for Maintenance Leak System Parts Leak System Parts ) ( & ' Figure 31 154 Leak system parts Item p/n Description 1 5061-3356 Leak sensor 2 G4226-44511 Leak plane 3 0890-1711 Leak tubing 185 mm 4 5041-8388 Leak funnel Agilent 1260 Infinity High Performance Autosampler User Manual
Agilent 1260 Infinity High Performance Autosampler User Manual 11 Identifying Cables Cable Overview 156 Analog Cables 158 Remote Cables 160 BCD Cables 163 CAN/LAN Cables 165 External Contact Cable Agilent Module to PC 166 167 Agilent 1200 Module to Printer 168 This chapter provides information on cables used with the Agilent 1200 Infinity Series modules.
11 Identifying Cables Cable Overview Cable Overview NOTE Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations.
Identifying Cables Cable Overview 11 CAN cables p/n Description 5181-1516 CAN cable, Agilent module to module, 0.5 m 5181-1519 CAN cable, Agilent module to module, 1 m LAN cables p/n Description 5023-0203 Cross-over network cable, shielded, 3 m (for point to point connection) 5023-0202 Twisted pair network cable, shielded, 7 m (for point to point connection) RS-232 cables p/n Description G1530-60600 RS-232 cable, 2 m RS232-61601 RS-232 cable, 2.5 m Instrument to PC, 9-to-9 pin (female).
11 Identifying Cables Analog Cables Analog Cables One end of these cables provides a BNC connector to be connected to Agilent modules. The other end depends on the instrument to which connection is being made.
Identifying Cables Analog Cables 11 Agilent Module to General Purpose p/n 01046-60105 Pin Pin Agilent module 1 Signal Name Not connected 2 Black Analog - 3 Red Analog + Agilent 1260 Infinity High Performance Autosampler User Manual 159
11 Identifying Cables Remote Cables Remote Cables One end of these cables provides a Agilent Technologies APG (Analytical Products Group) remote connector to be connected to Agilent modules. The other end depends on the instrument to be connected to.
Identifying Cables Remote Cables 11 Agilent Module to 3396 Series III / 3395B Integrators p/n 03396-61010 Pin 33XX Pin Agilent module Signal Name 9 1 - White Digital ground NC 2 - Brown Prepare run Low 3 3 - Gray Start Low NC 4 - Blue Shut down Low NC 5 - Pink Not connected NC 6 - Yellow Power on High 14 7 - Red Ready High 4 8 - Green Stop Low NC 9 - Black Start request Low 13, 15 Active (TTL) Not connected Agilent Module to Agilent 35900 A/D Converters p/n 5061-33
11 Identifying Cables Remote Cables Agilent Module to General Purpose p/n 01046-60201 162 Wire Color Pin Agilent module Signal Name Active (TTL) White 1 Digital ground Brown 2 Prepare run Low Gray 3 Start Low Blue 4 Shut down Low Pink 5 Not connected Yellow 6 Power on High Red 7 Ready High Green 8 Stop Low Black 9 Start request Low Agilent 1260 Infinity High Performance Autosampler User Manual
Identifying Cables BCD Cables 11 BCD Cables One end of these cables provides a 15- pin BCD connector to be connected to the Agilent modules.
11 Identifying Cables BCD Cables Agilent Module to 3396 Integrators p/n 03396-60560 164 Pin 3396 Pin Agilent module Signal Name BCD Digit 1 1 BCD 5 20 2 2 BCD 7 80 3 3 BCD 6 40 4 4 BCD 4 10 5 5 BCD0 1 6 6 BCD 3 8 7 7 BCD 2 4 8 8 BCD 1 2 9 9 Digital ground NC 15 +5V Low Agilent 1260 Infinity High Performance Autosampler User Manual
Identifying Cables CAN/LAN Cables 11 CAN/LAN Cables Both ends of this cable provide a modular plug to be connected to Agilent modules CAN or LAN connectors. CAN Cables p/n Description 5181-1516 CAN cable, Agilent module to module, 0.
11 Identifying Cables External Contact Cable External Contact Cable 5 10 15 1 6 11 One end of this cable provides a 15- pin plug to be connected to Agilent modules interface board. The other end is for general purpose.
Identifying Cables Agilent Module to PC 11 Agilent Module to PC p/n Description G1530-60600 RS-232 cable, 2 m RS232-61601 RS-232 cable, 2.5 m Instrument to PC, 9-to-9 pin (female). This cable has special pin-out, and is not compatible with connecting printers and plotters. It's also called "Null Modem Cable" with full handshaking where the wiring is made between pins 1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9.
11 Identifying Cables Agilent 1200 Module to Printer Agilent 1200 Module to Printer 168 p/n Description 5181-1529 Cable Printer Serial & Parallel, is a SUB-D 9 pin female vs. Centronics connector on the other end (NOT FOR FW UPDATE). For use with G1323 Control Module.
Agilent 1260 Infinity High Performance Autosampler User Manual 12 Hardware Information Firmware Description 170 Boot-up and Initialization Process 173 Electrical Connections 174 Rear view of the module 175 Interfaces 176 Overview Interfaces 179 Setting the 8-bit Configuration Switch 183 Communication Settings for RS-232C 184 Special Settings 186 Instrument Layout 187 Early Maintenance Feedback 188 This chapter describes the autosampler in more detail on hardware and electronics.
12 Hardware Information Firmware Description Firmware Description The firmware of the instrument consists of two independent sections: • a non- instrument specific section, called resident system • an instrument specific section, called main system Resident System This resident section of the firmware is identical for all Agilent 1100/1200/1220/1260/1290 series modules.
Hardware Information Firmware Description 12 Firmware Updates Firmware updates can be done using your user interface: • PC and Firmware Update Tool with local files on the hard disk • Instant Pilot (G4208A) with files from a USB Flash Disk • Agilent Lab Advisor software B.01.03 and above The file naming conventions are: PPPP_RVVV_XXX.
12 Hardware Information Firmware Description NOTE Some modules are limited in downgrading due to their main board version or their initial firmware revision. For example, a G1315C DAD SL cannot be downgraded below firmware revision B.01.02 or to a A.xx.xx. Some modules can be re-branded (e.g. G1314C to G1314B) to allow operation in specific control software environments. In this case the feature set of the target type are use and the feature set of the original are lost. After re-branding (e.g.
Hardware Information Boot-up and Initialization Process 12 Boot-up and Initialization Process CAUTION Obstruction of transport unit Any obstruction of the transport unit during the initialization process will result in a wrong transmission ratio and thus wrong needle positions. ➔ Make sure no vials or other material gets into the X-slide. 1 Firmware Boot Process. a Start Boot Loader. b Boot main firmware. OR Boot resident firmware (if set in VRAM, by DIP switch or if no/wrong main FW is found).
12 Hardware Information Electrical Connections Electrical Connections • The CAN bus is a serial bus with high speed data transfer. The two connectors for the CAN bus are used for internal module data transfer and synchronization. • One analog output provides signals for integrators or data handling systems. • The REMOTE connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features such as start, stop, common shut down, prepare, and so on.
Hardware Information Electrical Connections 12 Rear view of the module Figure 33 Rear view of the module Agilent 1260 Infinity High Performance Autosampler User Manual 175
12 Hardware Information Interfaces Interfaces The Agilent 1200 Infinity Series modules provide the following interfaces: Table 8 Agilent 1200 Infinity Series Interfaces Module CAN LAN/BCD (optional) LAN (on-board) RS-232 Analog APG Remote Special G1310B Iso Pump G1311B Quat Pump G1311C Quat Pump VL G1312B Bin Pump K1312B Bin Pump Clinical Ed.
Hardware Information Interfaces Table 8 12 Agilent 1200 Infinity Series Interfaces Module CAN LAN/BCD (optional) LAN (on-board) RS-232 Analog APG Remote Special G1314B VWD VL G1314C VWD VL+ 2 Yes No Yes 1 Yes G1314E/F VWD K1314F Clinical Ed. 2 No Yes Yes 1 Yes G4212A/B DAD K4212B DAD Clinical Ed. 2 No Yes Yes 1 Yes G1315C DAD VL+ G1365C MWD G1315D DAD VL G1365D MWD VL 2 No Yes Yes 2 Yes G1321B FLD K1321B FLD Clinical Ed.
12 Hardware Information Interfaces Table 8 Agilent 1200 Infinity Series Interfaces Module CAN LAN/BCD (optional) LAN (on-board) RS-232 Analog APG Remote Special G4227A Flex Cube 2 No No No No No CAN-DC- OUT for CAN slaves 1 G4240A CHIP CUBE 1 2 Yes No Yes No Yes CAN-DC- OUT for CAN slaves THERMOSTAT for G1330A/B (NOT USED), K1330B Requires a HOST module with on-board LAN (e.g. G4212A or G4220A with minimum firmware B.06.40 or C.06.
12 Hardware Information Interfaces Overview Interfaces CAN The CAN is inter- module communication interface. It is a 2- wire serial bus system supporting high speed data communication and real- time requirement. LAN The modules have either an interface slot for an LAN card (e.g. Agilent G1369B/C LAN Interface) or they have an on- board LAN interface (e.g. detectors G1315C/D DAD and G1365C/D MWD). This interface allows the control of the module/system via a PC with the appropriate control software.
12 Hardware Information Interfaces The RS- 232C is designed as DCE (data communication equipment) with a 9- pin male SUB- D type connector. The pins are defined as: Table 9 RS-232C Connection Table Pin Direction Function 1 In DCD 2 In RxD 3 Out TxD 4 Out DTR 5 Ground 6 In DSR 7 Out RTS 8 In CTS 9 In RI >chigjbZci BVaZ Figure 34 E8 ;ZbVaZ ;ZbVaZ BVaZ RS-232 Cable Analog Signal Output The analog signal output can be distributed to a recording device.
Hardware Information Interfaces 12 APG Remote The APG Remote connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down, prepare, and so on. Remote control allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements. The subminiature D connector is used. The module provides one remote connector which is inputs/outputs (wired- or technique).
12 Hardware Information Interfaces Table 10 Remote Signal Distribution Pin Signal Description 1 DGND Digital ground 2 PREPARE (L) Request to prepare for analysis (for example, calibration, detector lamp on). Receiver is any module performing pre-analysis activities. 3 START (L) Request to start run / timetable. Receiver is any module performing run-time controlled activities. 4 SHUT DOWN (L) System has serious problem (for example, leak: stops pump).
Hardware Information Setting the 8-bit Configuration Switch 12 Setting the 8-bit Configuration Switch The 8- bit configuration switch is located at the rear of the module. This module does not have its own on- board LAN interface. It can be controlled through the LAN interface of another module, and a CAN connection to that module.
12 Hardware Information Setting the 8-bit Configuration Switch NOTE With the introduction of the Agilent 1260 Infinity, all GPIB interfaces have been removed. The preferred communication is LAN. NOTE The following tables represent the configuration switch settings for the modules without on-board LAN only.
Hardware Information Setting the 8-bit Configuration Switch 12 Use the following tables for selecting the setting which you want to use for RS- 232C communication. The number 0 means that the switch is down and 1 means that the switch is up.
12 Hardware Information Setting the 8-bit Configuration Switch Special Settings The special settings are required for specific actions (normally in a service case). Boot-Resident Firmware update procedures may require this mode in case of firmware loading errors (main firmware part). If you use the following switch settings and power the instrument up again, the instrument firmware stays in the resident mode. It is not operable as a module.
Hardware Information Instrument Layout 12 Instrument Layout The industrial design of the module incorporates several innovative features. It uses Agilent’s E- PAC concept for the packaging of electronics and mechanical assemblies. This concept is based upon the use of expanded polypropylene (EPP) layers of foam plastic spacers in which the mechanical and electronic boards components of the module are placed.
12 Hardware Information Early Maintenance Feedback Early Maintenance Feedback Maintenance requires the exchange of components which are subject to wear or stress. Ideally, the frequency at which components are exchanged should be based on the intensity of usage of the module and the analytical conditions, and not on a predefined time interval.
Agilent 1260 Infinity High Performance Autosampler User Manual 13 LAN Configuration Setting up the module in a LAN environment Connecting the module via LAN 190 191 This chapter provides information on connecting the autosampler to the Agilent ChemStation PC.
13 LAN Configuration Setting up the module in a LAN environment Setting up the module in a LAN environment It is not recommended to connect an Agilent 1260 Infinity system via the G1367E Autosampler. The detector is producing the most data in the stack, followed by the pump, and it is therefore highly recommended to use either of these modules for the LAN connection.
LAN Configuration Connecting the module via LAN 13 Connecting the module via LAN If the module is being operated as a standalone module or if a connection via LAN is required regardless of above mentioned recommendation, a G1369B/C LAN card has to be used. For installation and configuration, see the G1369B/C documentation.
13 LAN Configuration Connecting the module via LAN 192 Agilent 1260 Infinity High Performance Autosampler User Manual
Agilent 1260 Infinity High Performance Autosampler User Manual 14 Appendix General Safety Information Lithium Batteries Information 194 197 The Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC) 198 Radio Interference 199 Sound Emission 200 Use of Solvents 201 Agilent Technologies on Internet 202 This chapter provides addition information on safety, legal and web.
14 Appendix General Safety Information General Safety Information General Safety Information The following general safety precautions must be observed during all phases of operation, service, and repair of this instrument. Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design, manufacture, and intended use of the instrument. Agilent Technologies assumes no liability for the customer’s failure to comply with these requirements.
14 Appendix General Safety Information Operation Before applying power, comply with the installation section. Additionally the following must be observed. Do not remove instrument covers when operating. Before the instrument is switched on, all protective earth terminals, extension cords, auto- transformers, and devices connected to it must be connected to a protective earth via a ground socket.
14 Appendix General Safety Information Safety Symbols Table 18 Safety Symbols Symbol Description The apparatus is marked with this symbol when the user should refer to the instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage. Indicates dangerous voltages. Indicates a protected ground terminal. Indicates eye damage may result from directly viewing the light produced by the deuterium lamp used in this product.
Appendix Lithium Batteries Information 14 Lithium Batteries Information WA R N I N G Lithium batteries may not be disposed-off into the domestic waste. Transportation of discharged Lithium batteries through carriers regulated by IATA/ICAO, ADR, RID, IMDG is not allowed. Danger of explosion if battery is incorrectly replaced. ➔ Discharged Lithium batteries shall be disposed off locally according to national waste disposal regulations for batteries.
14 Appendix The Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC) The Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC) Abstract The Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC), adopted by EU Commission on 13 February 2003, is introducing producer responsibility on all Electric and Electronic appliances from 13 August 2005. NOTE This product complies with the WEEE Directive (2002/96/EC) marking requirements.
Appendix Radio Interference 14 Radio Interference Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations. Test and Measurement If test and measurement equipment is operated with equipment unscreened cables and/or used for measurements on open set- ups, the user has to assure that under operating conditions the radio interference limits are still met within the premises.
14 Appendix Sound Emission Sound Emission Manufacturer’s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991. This product has a sound pressure emission (at the operator position) < 70 dB.
Appendix Use of Solvents 14 Use of Solvents Observe the following recommendations on the use of solvents. • Brown glass ware can avoid growth of algae.
14 Appendix Agilent Technologies on Internet Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at: http://www.agilent.
Index Index 8 8-bit configuration switch without On-Board LAN 183 A accessory kit 34 Agilent Lab Advisor software 84 Agilent Lab Advisor 84 Agilent on internet 202 algae 201 ambient non-operating temperature 27 ambient operating temperature 27 analog signal 180 analog cable 158 apg remote 181 arm 114 position 114 automated delay volume reduction 77 B battery safety information BCD cable 163 bench space 26 197 connecting APG remote 39 connecting CAN 39 connecting LAN 39 connecting the ChemStation 39 c
Index external contact cable 166 extra-column volume 66 F fan failed 92 firmware description 170 main system 170 resident system 170 update tool 171 updates 171, 144, 144 upgade/downgrade 144 upgrade/downgrade 144 frequency range 27 G general error messages 88 H humidity 27 I injection volume achieving higher volumes 70 injector steps 116 installation bench space 26 power considerations 24 installing metering seal 138 instrument layout 187 interfaces 176 internet 202 L LAN 204 cable 165 leak sens
Index S V safety class I 194 safety information lithium batteries 197 safety general information 194 standards 27 symbols 196 sensitivity optimization 76 shutdown 88 site requirements power cords 25 solvents 201 special interfaces 182 special settings boot-resident 186 forced cold start 186 specification physical 27 stack configuration front view 38 rear view 39 status indicator 82 steps injector 116 step commands 117 system setup and installation optimizing stack configuration vial trays 147 voltage ra
www.agilent.com In This Book This manual contains technical reference information about the Agilent 1260 Infinity High Performance Autosampler G1367E. • introduction and specifications, • installation, • using and optimizing, • troubleshooting and diagnose, • maintenance, • parts identification, • safety and related information. © Agilent Technologies 2010-2013, 2014 Printed in Germany 02/2014 *G1367-90014* *G1367-90014* G1367-90014 Rev.
