Technical data

146 Understanding Your Agilent ChemStation

7 Calibration

Unknown Samples

An unknown sample is a sample containing an unknown amount of the

compound to be quantified.

To find out how much of the compound is in the unknown sample, you must:

• create a calibration curve for the compound,

• inject an aliquot of your unknown sample and run the analysis in exactly

the same way as for the calibration sample,

• determine from the signal the response, which is the area or height of the

peak due to the unknown amount of the compound, and

• use the calibration curve to calculate the amount of the compound in the

unknown sample.

For example, if the area of peak in the unknown sample is 500, you can

determine that the amount in the unknown is 5 ng/µl, by using the calibration

curve shown in Figure 33 on page 146.

Figure 33 Signal from Unknown Sample and Calibration Curve

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